ArticleBMC plant biology2009
Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin.
Article in BMC plant biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Antibodies to the wheat storage globulin Glo-3A in children before and at diagnosis of celiac disease.Journal of pediatric gastroenterology and nutrition · 2011Trial
- Biochemical Analysis of Wheat Milling By-Products for Their Valorization as Potential Food Ingredients.International journal of molecular sciences · 2025Article
- The Impact of Diet Wheat Source on the Onset of Type 1 Diabetes Mellitus-Lessons Learned from the Non-Obese Diabetic (NOD) Mouse Model.Nutrients · 2017Article
- Diagonal two-dimensional electrophoresis (D-2DE): a new approach to study the effect of osmotic stress induced by polyethylene glycol in durum wheat (Triticum durum Desf.).Molecular biology reports · 2016Article
- Teff, an Orphan Cereal in the Chloridoideae, Provides Insights into the Evolution of Storage Proteins in Grasses.Genome biology and evolution · 2016Article
- Proteomic analysis of albumins and globulins from wheat variety Chinese Spring and its fine deletion line 3BS-8.International journal of molecular sciences · 2012Article
- Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos.BMC research notes · 2012Article
- Animal models to study gluten sensitivity.Seminars in immunopathology · 2012Review
- Antibodies from a patient with type 1 diabetes and celiac disease bind to macrophages that express the scavenger receptor CD163.Canadian journal of gastroenterology = Journal canadien de gastroenterologie · 2011Article
- Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry.Proteome science · 2011Article
- Brachypodium distachyon grain: identification and subcellular localization of storage proteins.Journal of experimental botany · 2010Article
- Peripherin-reactive antibodies in mouse, rabbit, and human blood.Journal of proteome research · 2010Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExposure to dietary wheat proteins in genetically susceptible individuals has been associated with increased risk for the development of Type 1 diabetes (T1D). Recently, a wheat protein encoded by cDNA WP5212 has been shown to be antigenic in mice, rats and humans with autoimmune T1D. To investigate the genomic origin of the identified wheat protein cDNA, a hexaploid wheat genomic library from Glenlea cultivar was screened.
resultsThree unique wheat globulin genes, Glo-3A, Glo3-B and Glo-3C, were identified. We describe the genomic structure of these genes and their expression pattern in wheat seeds. The Glo-3A gene shared 99% identity with the cDNA of WP5212 at the nucleotide and deduced amino acid level, indicating that we have identified the gene(s) encoding wheat protein WP5212. Southern analysis revealed the presence of multiple copies of Glo-3-like sequences in all wheat samples, including hexaploid, tetraploid and diploid species wheat seed. Aleurone and embryo tissue specificity of WP5212 gene expression, suggested by promoter region analysis, which demonstrated an absence of endosperm specific cis elements, was confirmed by immunofluorescence microscopy using anti-WP5212 antibodies.
conclusionTaken together, the results indicate that a diverse group of globulins exists in wheat, some of which could be associated with the pathogenesis of T1D in some susceptible individuals. These data expand our knowledge of specific wheat globulins and will enable further elucidation of their role in wheat biology and human health.
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