ArticleCellular microbiology2009
Ubiquitination of the bacterial inositol phosphatase, SopB, regulates its biological activity at the plasma membrane.
Article in Cellular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 73 citations in OpenAlex.
- Molecular strategies ofFrontiers in cellular and infection microbiology · 2026Review
- Enzymatic versatility of the salmonella effector SopB enables manipulation of host phosphoinositide signalling.Discover bacteria · 2026Review
- Review
- Ubiquitin and its relatives as wizards of the endolysosomal system.Journal of cell science · 2023Article
- Manipulation of host immune defenses by effector proteins delivered from multiple secretion systems ofFrontiers in immunology · 2023Review
- Article
- Global mapping of Salmonella enterica-host protein-protein interactions during infection.Cell host & microbe · 2021Article
- Ehrlichia TRP effectors: moonlighting, mimicry and infection.Pathogens and disease · 2021Review
- A pipeline to evaluate inhibitors of the Pseudomonas aeruginosa exotoxin U.The Biochemical journal · 2021Article
- Ehrlichia chaffeensis TRP120-mediated ubiquitination and proteasomal degradation of tumor suppressor FBW7 increases oncoprotein stability and promotes infection.PLoS pathogens · 2020Article
- Ubiquitination ofmBio · 2019Article
- SopF, a phosphoinositide binding effector, promotes the stability of the nascent Salmonella-containing vacuole.PLoS pathogens · 2019Article
- Chloroplasts at the Crossroad of Photosynthesis, Pathogen Infection and Plant Defense.International journal of molecular sciences · 2018Review
- Ubiquitin, SUMO, and NEDD8: Key Targets of Bacterial Pathogens.Trends in cell biology · 2018Review
- Controlled Activity of themBio · 2017Article
- Ehrlichia chaffeensis TRP120 Moonlights as a HECT E3 Ligase Involved in Self- and Host Ubiquitination To Influence Protein Interactions and Stability for Intracellular Survival.Infection and immunity · 2017Article
- Exploitation of the host cell ubiquitin machinery by microbial effector proteins.Journal of cell science · 2017Review
- A second wave of Salmonella T3SS1 activity prolongs the lifespan of infected epithelial cells.PLoS pathogens · 2017Article
- Methods to Illuminate the Role ofFrontiers in cellular and infection microbiology · 2017Review
- Predictable, Tunable Protein Production inFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The Salmonella type III effector, SopB, is an inositol polyphosphate phosphatase that modulates host cell phospholipids at the plasma membrane and the nascent Salmonella-containing vacuole (SCV). Translocated SopB persists for many hours after infection and is ubiquitinated but the significance of this covalent modification has not been investigated. Here we identify by mass spectrometry six lysine residues of SopB that are mono-ubiquitinated. Substitution of these six lysine residues with arginine, SopB-K(6)R, almost completely eliminated SopB ubiquitination. We found that ubiquitination does not affect SopB stability or membrane association, or SopB-dependent events in SCV biogenesis. However, two spatially and temporally distinct events are dependent on ubiquitination, downregulation of SopB activity at the plasma membrane and prolonged retention of SopB on the SCV. Activation of the mammalian pro-survival kinase Akt/PKB, a downstream target of SopB, was intensified and prolonged after infection with the SopB-K(6)R mutant. At later times, fewer SCV were decorated with SopB-K(6)R compared with SopB. Instead SopB-K(6)R was present as discrete vesicles spread diffusely throughout the cell. Altogether, our data show that ubiquitination of SopB is not related to its intracellular stability but rather regulates its enzymatic activity at the plasma membrane and intracellular localization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.