ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2009
Quantitative mechanics of endothelial phagocytosis of silicon microparticles.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Cuboids Prevail When Unraveling the Influence of Microchip Geometry on Macrophage Interactions and Metabolic Responses.ACS biomaterials science & engineering · 2024Article
- Differential Protein Adsorption and Cellular Uptake of Silica Nanoparticles Based on Size and Porosity.ACS applied materials & interfaces · 2016Article
- Ultrapure laser-synthesized Si-based nanomaterials for biomedical applications: in vivo assessment of safety and biodistribution.Scientific reports · 2016Article
- A specifically designed nanoconstruct associates, internalizes, traffics in cardiovascular cells, and accumulates in failing myocardium: a new strategy for heart failure diagnostics and therapeutics.European journal of heart failure · 2016Article
- Formation of a protein corona on silver nanoparticles mediates cellular toxicity via scavenger receptors.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
- Multistage Nanovectors Enhance the Delivery of Free and Encapsulated Drugs.Current drug targets · 2015Article
- Polymeric stent materials dysregulate macrophage and endothelial cell functions: implications for coronary artery stent.International journal of cardiology · 2014Article
- Cellular communication via nanoparticle-transporting biovesicles.Nanomedicine (London, England) · 2014Article
- Porous silicon advances in drug delivery and immunotherapy.Current opinion in pharmacology · 2013Review
- Comparison of nanotube-protein corona composition in cell culture media.Small (Weinheim an der Bergstrasse, Germany) · 2013Article
- Short and long term, in vitro and in vivo correlations of cellular and tissue responses to mesoporous silicon nanovectors.Small (Weinheim an der Bergstrasse, Germany) · 2013Article
- Silicon micro- and nanofabrication for medicine.Advanced healthcare materials · 2013Review
- Inter-endothelial transport of microvectors using cellular shuttles and tunneling nanotubes.Small (Weinheim an der Bergstrasse, Germany) · 2012Article
- Brain microvascular endothelial cell association and distribution of a 5 nm ceria engineered nanomaterial.International journal of nanomedicine · 2012Article
- Preparation, characterization, and cellular associations of silicon logic-embedded vectors.Methods in enzymology · 2012Article
- Physiologically based pharmacokinetic modeling of PLGA nanoparticles with varied mPEG content.International journal of nanomedicine · 2012Article
- Multi-stage delivery nano-particle systems for therapeutic applications.Biochimica et biophysica acta · 2011Review
- Near-infrared imaging method for the in vivo assessment of the biodistribution of nanoporous silicon particles.Molecular imaging · 2011Article
- In vivo evaluation of safety of nanoporous silicon carriers following single and multiple dose intravenous administrations in mice.International journal of pharmaceutics · 2010Article
- Logic-embedded vectors for intracellular partitioning, endosomal escape, and exocytosis of nanoparticles.Small (Weinheim an der Bergstrasse, Germany) · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Endothelia, once thought of as a barrier to the delivery of therapeutics, is now a major target for tissue-specific drug delivery. Tissue- and disease-specific molecular presentations on endothelial cells provide targets for anchoring or internalizing delivery vectors. Porous silicon delivery vectors are phagocytosed by vascular endothelial cells. The rapidity and efficiency of silicon microparticle uptake lead us to delineate the kinetics of internalization. To discriminate between surface-attached and -internalized microparticles, we developed a double fluorescent/FRET flow cytometric approach. The approach relies on quenching of antibody-conjugated fluorescein isothiocyanate covalently attached to the microparticle surface by attachment of a secondary antibody labeled with an acceptor fluorophore, phycoerythrin. The resulting half-time for microparticle internalization was 15.7 min, with confirmation provided by live confocal imaging as well as transmission electron microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.