ArticleInvestigative ophthalmology & visual science2009
Differential gene expression in the pig limbal side population: implications for stem cell cycling, replication, and survival.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Hyaluronan supports the limbal stem cell phenotype during ex vivo culture.Stem cell research & therapy · 2022Article
- LNX1/LNX2 proteins: functions in neuronal signalling and beyond.Neuronal signaling · 2018Review
- Comparison of culture media indicates a role for autologous serum in enhancing phenotypic preservation of rabbit limbal stem cells in explant culture.Cytotechnology · 2018Article
- Characterisation of human limbal side population cells isolated using an optimised protocol from an immortalised epithelial cell line and primary limbal cultures.Stem cell reviews and reports · 2014Article
- Ocular surface development and gene expression.Journal of ophthalmology · 2013Article
- Preferential biological processes in the human limbus by differential gene profiling.PloS one · 2013Article
- Transcriptional dissection of human limbal niche compartments by massive parallel sequencing.PloS one · 2013Article
- Stem cells and corneal epithelial maintenance: insights from the mouse and other animal models.Results and problems in cell differentiation · 2012Review
- ABCG2-dependent dye exclusion activity and clonal potential in epithelial cells continuously growing for 1 month from limbal explants.Investigative ophthalmology & visual science · 2011Article
- A comparison of stem cell-related gene expression in the progenitor-rich limbal epithelium and the differentiating central corneal epithelium.Molecular vision · 2011Article
- Gene expression analysis in SV-40 immortalized human corneal epithelial cells cultured with an air-liquid interface.Molecular vision · 2010Article
- Comparative transcriptional profiling of the limbal epithelial crypt demonstrates its putative stem cell niche characteristics.BMC genomics · 2010Article
- DUSP5 and DUSP6 modulate corneal epithelial cell proliferation.Molecular vision · 2010Article
- Gene expression profile of epithelial cells and mesenchymal cells derived from limbal explant culture.Molecular vision · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo define the molecular signature of limbal SP cells and identify signaling pathways associated with the phenotype of these putative stem cells.
methodsPrimary cultures of pig limbal epithelial cells stained with Hoechst 33342 were sorted by flow cytometry into SP and non-SP cells, and purified RNA was processed for microarray analysis with an oligonucleotide spotted array. Expressed transcripts for which SP and non-SP expressions differed by more that 1.5-fold in each paired set and by twofold overall were considered to be differentially expressed. Differential expression was validated by quantitative PCR and immunostaining. Data-mining methods were used to identify cellular processes that are either salient or depressed in the SP cells.
resultsThe microarray identified approximately 9000 distinct, expressed, and identifiable genes. Of those, 382 and 296 were either over- or underexpressed in the SP cells, respectively. Overrepresentation analysis indicated that SP cells are in a low metabolic and biosynthetic state. In addition, a pattern of elevated MXD1, MAXI2, DUSP5, p27/KIP1, and p57/KIP2 and decreased Cyclin D and CDK genes can be expected to slow intrinsic and mitogen-induced G(1)-to-S cell cycle transition. SP cells were also rich in genes associated with stem cell phenotype and genes providing protection against oxidative and/or xenobiotic damage.
conclusionsMicroarray analysis of pig limbal SP cells yielded a molecular signature underscoring a phenotype characterized by slow cycling and low metabolic activity. The results provide valuable insights for the preservation and/or replication of epithelial stem cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.