ArticleInvestigative ophthalmology & visual science2009
Characterization of P2X7 purinergic receptors and their function in rat lacrimal gland.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Inflammasomes in Sjögren's Disease: Exploring the Therapeutic Value.International journal of molecular sciences · 2026Review
- Genomic DNA activates the AIM2 inflammasome and STING pathways to induce inflammation in lacrimal gland myoepithelial cells.The ocular surface · 2023Article
- Novel Insights Into Muscarinic and Purinergic Responses in Primary Cultures of Rat Lacrimal Gland Myoepithelial Cells.Investigative ophthalmology & visual science · 2021Article
- P2 Receptors as Therapeutic Targets in the Salivary Gland: From Physiology to Dysfunction.Frontiers in pharmacology · 2020Review
- The P2X7 receptor and pannexin-1 are involved in glucose-induced autocrine regulation in β-cells.Scientific reports · 2018Article
- TFOS DEWS II pain and sensation report.The ocular surface · 2017Review
- The Role of the P2X7 Receptor in Ocular Stresses: A Potential Therapeutic Target.Vision (Basel, Switzerland) · 2017Review
- Signaling Pathways of Purinergic Receptors and Their Interactions with Cholinergic and Adrenergic Pathways in the Lacrimal Gland.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2016Review
- Homodimeric anoctamin-1, but not homodimeric anoctamin-6, is activated by calcium increases mediated by the P2Y1 and P2X7 receptors.Pflugers Archiv : European journal of physiology · 2015Article
- Purines in the eye: recent evidence for the physiological and pathological role of purines in the RPE, retinal neurons, astrocytes, Müller cells, lens, trabecular meshwork, cornea and lacrimal gland.Experimental eye research · 2014Review
- Loss of P2X7 nucleotide receptor function leads to abnormal fat distribution in mice.Purinergic signalling · 2014Article
- P2Y purinoceptors induce changes in intracellular calcium in acinar cells of rat lacrimal glands.Histochemistry and cell biology · 2012Article
- Increase of intracellular Ca2+ by purinergic receptors in cultured rat lacrimal gland myoepithelial cells.Investigative ophthalmology & visual science · 2011Article
- Inhibition of the P2X7 receptor reduces cystogenesis in PKD.Journal of the American Society of Nephrology : JASN · 2011Article
- Interaction of alpha1D-adrenergic and P2X(7) receptors in the rat lacrimal gland and the effect on intracellular [Ca2+] and protein secretion.Investigative ophthalmology & visual science · 2011Article
- Identification of P2X₃ and P2X₇ purinergic receptors activated by ATP in rat lacrimal gland.Investigative ophthalmology & visual science · 2011Article
- Cholinergic agonists activate P2X7 receptors to stimulate protein secretion by the rat lacrimal gland.Investigative ophthalmology & visual science · 2011Article
- Effect of P2X(7) receptor knockout on exocrine secretion of pancreas, salivary glands and lacrimal glands.The Journal of physiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo characterize the effects of P2X(7) purinergic receptors on lacrimal gland function.
methodsP2X(7) purinergic receptors were identified by RT-PCR, Western blot analysis, and immunofluorescence techniques. Rat lacrimal gland acini were isolated by collagenase digestion. Acini were incubated with the fluorescent indicator molecule fura 2, and [Ca(2+)](i) was measured by a fluorescence imaging system. Protein secretion was measured with a fluorescence assay system. Activation of ERK 1/2 was determined by Western blot analysis with an antibody against phosphorylated (active) ERK 1/2.
resultsP2X(7) receptors were present in the lacrimal gland by RT-PCR and Western blot analysis. These receptors were located in the membranes of acinar and ductal cells and the cytoplasm of acinar cells. Activation of P2X(7) receptors with (benzoylbenzoyl)adenosine 5'-triphosphate increased [Ca(2+)](i), peroxidase secretion, and ERK 1/2 activation, each of which was inhibited by the P2X(7) receptor inhibitors Brilliant Blue G or A 438079.
conclusionsP2X(7) purinergic receptors are present in rat lacrimal gland and when stimulated increase [Ca(2+)](i), protein secretion, and ERK 1/2 activation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.