ArticleLab on a chip2009
Isolation, incubation, and parallel functional testing and identification by FISH of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement.
Article in Lab on a chip, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 98 citations in OpenAlex.
- Research progress in isolation and identification of rumen probiotics.Frontiers in cellular and infection microbiology · 2024Review
- Double emulsions as a high-throughput enrichment and isolation platform for slower-growing microbes.ISME communications · 2023Article
- [Application of Droplet-Based Microfluidics in Microbial Research].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2023Review
- Selective single-bacteria extraction based on capture and release of microemulsion droplets.Scientific reports · 2022Article
- Droplet Microfluidics for Microbial Biotechnology.Advances in biochemical engineering/biotechnology · 2022Article
- One cell at a time: droplet-based microbial cultivation, screening and sequencing.Marine life science & technology · 2021Review
- FISH Variants.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Interindividual Variation in Dietary Carbohydrate Metabolism by Gut Bacteria Revealed with Droplet Microfluidic Culture.mSystems · 2020Article
- FISH and chips: a review of microfluidic platforms for FISH analysis.Medical microbiology and immunology · 2020Review
- Rapid Microfluidic Mixer Based on Ferrofluid and Integrated Microscale NdFeB-PDMS Magnet.Micromachines · 2019Article
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- Simple and Precise Counting of Viable Bacteria by Resazurin-Amplified Picoarray Detection.Analytical chemistry · 2018Article
- A Microfluidics and Agent-Based Modeling Framework for Investigating Spatial Organization in Bacterial Colonies: The Case ofFrontiers in microbiology · 2018Article
- Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing.SLAS technology · 2017Article
- Accelerating bacterial growth detection and antimicrobial susceptibility assessment in integrated picoliter droplet platform.Biosensors & bioelectronics · 2017Article
- Stochastically reduced communities-Microfluidic compartments as model and investigation tool for soil microorganism growth in structured spaces.Engineering in life sciences · 2017Article
- Analysing Microbial Community Composition through Amplicon Sequencing: From Sampling to Hypothesis Testing.Frontiers in microbiology · 2017Review
- Single-cell barcoding and sequencing using droplet microfluidics.Nature protocols · 2017Article
- Biocompatibility of fluids for multiphase drops-in-drops microfluidics.Biomedical microdevices · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
This paper illustrates a plug-based microfluidic approach combining the technique of the chemistrode and the principle of stochastic confinement, which can be used to i) starting from a mixture of cells, stochastically isolate single cells into plugs, ii) incubate the plugs to grow clones of the individual cells without competition among different clones, iii) split the plugs into arrays of identical daughter plugs, where each plug contained clones of the original cell, and iv) analyze each array by an independent technique, including cellulase assays, cultivation, cryo-preservation, Gram staining, and Fluorescence In Situ Hybridization (FISH). Functionally, this approach is equivalent to simultaneously assaying the clonal daughter cells by multiple killing and non-killing methods. A new protocol for single-cell FISH, a killing method, was developed to identify isolated cells of Paenibacillus curdlanolyticus in one array of daughter plugs using a 16S rRNA probe, Pc196. At the same time, live copies of P. curdlanolyticus in another array were obtained for cultivation. Among technical advances, this paper reports a chemistrode that enables sampling of nanoliter volumes directly from environmental specimens, such as soil slurries. In addition, a method for analyzing plugs is described: an array of droplets is deposited on the surface, and individual plugs are injected into the droplets of the surface array to induce a reaction and enable microscopy without distortions associated with curvature of plugs. The overall approach is attractive for identifying rare, slow growing microorganisms and would complement current methods to cultivate unculturable microbes from environmental samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.