ArticlePloS one2009
Nanomechanical in situ monitoring of proteolysis of peptide by Cathepsin B.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Fabricating Silicon Resonators for Analysing Biological Samples.Micromachines · 2021Review
- Blood Droplet-Based Cancer Diagnosis via Proteolytic Activity Measurement in Cancer Progression.Theranostics · 2017Article
- Tunable micro- and nanomechanical resonators.Sensors (Basel, Switzerland) · 2015Review
- Experimental and computational characterization of biological liquid crystals: a review of single-molecule bioassays.International journal of molecular sciences · 2009Review
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Authors and funding
10 authors.
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Abstract
Characterization and control of proteolysis of peptides by specific cellular protease is a priori requisite for effective drug discovery. Here, we report the nanomechanical, in situ monitoring of proteolysis of peptide chain attributed to protease (Cathepsin B) by using a resonant nanomechanical microcantilever immersed in a liquid. Specifically, the detection is based on measurement of resonant frequency shift arising from proteolysis of peptides (leading to decrease of cantilever's overall mass, and consequently, increases in the resonance). It is shown that resonant microcantilever enables the quantification of proteolysis efficacy with respect to protease concentration. Remarkably, the nanomechanical, in situ monitoring of proteolysis allows us to gain insight into the kinetics of proteolysis of peptides, which is well depicted by Langmuir kinetic model. This implies that nanomechanical biosensor enables the characterization of specific cellular protease such as its kinetics.
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