ArticleMolecular biology of the cell2009
The insulin-like growth factor (IGF)-I E-peptides modulate cell entry of the mature IGF-I protein.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Insulin-like Growth Factor 1 (IGF1) and Its Isoforms: Insights into the Mechanisms of Endometrial Cancer.Cancers · 2025Review
- Single-cell profiling of the amphioxus digestive tract reveals conservation of endocrine cells in chordates.Science advances · 2024Article
- Ablation of specific insulin-like growth factor I forms reveals the importance of cleavage for regenerative capacity and glycosylation for skeletal muscle storage.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Molecular cloning, expression, and functional features of IGF1 splice variants in sheep.Endocrine connections · 2021Article
- Insulin-Like Growth Factor-1 (IGF-1) and Its Monitoring in Medical Diagnostic and in Sports.Biomolecules · 2021Review
- Role of Alternatively Spliced Messenger RNA (mRNA) Isoforms of the Insulin-Like Growth Factor 1 (IGF1) in Selected Human Tumors.International journal of molecular sciences · 2020Review
- Overexpression of Mechano-Growth Factor Modulates Inflammatory Cytokine Expression and Macrophage Resolution in Skeletal Muscle Injury.Frontiers in physiology · 2018Article
- MUC4 is negatively regulated through the Wnt/β-catenin pathway via the Notch effector Hath1 in colorectal cancer.Genes & cancer · 2016Article
- IGF expression in HPV-related and HPV-unrelated human cancer cells.Oncology reports · 2014Article
- The complexity of the IGF1 gene splicing, posttranslational modification and bioactivity.Molecular medicine (Cambridge, Mass.) · 2014Review
- Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.American journal of physiology. Endocrinology and metabolism · 2014Article
- Matrix metalloproteinase 13 is a new contributor to skeletal muscle regeneration and critical for myoblast migration.American journal of physiology. Cell physiology · 2013Article
- The pro-forms of insulin-like growth factor I (IGF-I) are predominant in skeletal muscle and alter IGF-I receptor activation.Endocrinology · 2013Article
- Complexin activates exocytosis of distinct secretory vesicles controlled by different synaptotagmins.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Evidence for the Possible Biological Significance of the igf-1 Gene Alternative Splicing in Prostate Cancer.Frontiers in endocrinology · 2013Article
- New Modulators for IGF-I Activity within IGF-I Processing Products.Frontiers in endocrinology · 2013Article
- IGF-I 3' untranslated region: strain-specific polymorphisms and motifs regulating IGF-I in osteoblasts.Endocrinology · 2013Article
- Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Article
- E-peptides control bioavailability of IGF-1.PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Insulin-like growth factor (IGF)-I is a critical protein for cell development and growth. Alternative splicing of the igf1 gene gives rise to multiple isoforms. In rodents, proIGF-IA and proIGF-IB have different carboxy-terminal extensions called the E-peptides (EA and EB) and upon further posttranslational processing, produce the identical mature IGF-I protein. Rodent EB has been reported to have mitogenic and motogenic effects independent of IGF-I. However, effects of EA or EB on mature IGF-I, or whether proIGF-IA and proIGF-IB have different properties, have not been addressed. To determine whether the presence of EA or EB affected the distribution and stability of mature IGF-I protein, transient transfections of cDNAs encoding murine IGF-IA, IGF-IB, and mature IGF-I were performed in C2C12 cells, a skeletal muscle cell line. IGF-I secretion was measured by enzyme-linked immunosorbent assay of the media, and did not differ between expression of proIGF-IA, proIGF-IB, or mature IGF-I expression. Next, epitope-tagged constructs were transfected to determine cellular distribution of IGF-I, EA, and EB in the cells throughout the culture. IGF-I was detected in significantly fewer nontransfected cells in cultures transfected with mature IGF-I compared with transfection of proIGF-IA or proIGF-IB. These results demonstrate that EA and EB are not required for IGF-I secretion but that they increase cell entry of IGF-I from the media. This study provides evidence that the EA and EB may modulate IGF-I in addition to having independent activity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.