ArticleJournal of virology2009
Hepatitis C virus core protein and cellular protein HAX-1 promote 5-fluorouracil-mediated hepatocyte growth inhibition.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Molecular functions of HAX1 during disease progress.Virus genes · 2024Review
- Knockdown of Long Noncoding RNA 01124 Inhibits the Malignant Behaviors of Colon Cancer Cells via miR-654-5p/HAX-1.Evidence-based complementary and alternative medicine : eCAM · 2022Article
- Luciferase-Based Biosensors in the Era of the COVID-19 Pandemic.ACS nanoscience Au · 2021Review
- A Human Endogenous Bornavirus-Like Nucleoprotein Encodes a Mitochondrial Protein Associated with Cell Viability.Journal of virology · 2021Article
- MicroRNA‑125a‑5p regulates liver cancer cell growth, migration and invasion and EMT by targeting HAX1.International journal of molecular medicine · 2020Article
- Intrinsically disordered HAX-1 regulates CaBiochimica et biophysica acta. Biomembranes · 2020Article
- Role of the tumour protein P53 gene in human cervical squamous carcinoma cells: Discussing haematopoietic cell-specific protein 1-associated protein X-1-induced survival, migration and proliferation.Oncology letters · 2018Article
- Identification of the Functional Autophagy-Regulatory Domain in HCLS1-Associated Protein X-1 That Resists Against Oxidative Stress.DNA and cell biology · 2018Article
- Characterization of Recombinant Flaviviridae Viruses Possessing a Small Reporter Tag.Journal of virology · 2018Article
- High expression of HAX-1 protein is associated with tumor growth in papillary thyroid carcinoma.International journal of clinical and experimental pathology · 2018Article
- Hepatitis C virus Core overcomes all-Oncotarget · 2017Article
- Clinical and biological significance of HAX-1 overexpression in nasopharyngeal carcinoma.Oncotarget · 2016Article
- Expression of HAX-1 in colorectal cancer and its role in cancer cell growth.Molecular medicine reports · 2015Article
- HAX-1 is overexpressed in hepatocellular carcinoma and promotes cell proliferation.International journal of clinical and experimental pathology · 2015Article
- Expression and Function of HAX-1 in Human Cutaneous Squamous Cell Carcinoma.Journal of Cancer · 2015Article
- Hepatitis C virus impairs natural killer cell-mediated augmentation of complement synthesis.Journal of virology · 2014Article
- Expression of HAX-1 in human colorectal cancer and its clinical significance.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Role of the exogenous HCV core protein in the interaction of human hepatocyte proliferation and macrophage sub-populations.PloS one · 2014Article
- Analysis of HAX-1 gene expression in esophageal squamous cell carcinoma.Diagnostic pathology · 2013Article
- Oncogenic potential of hepatitis C virus proteins.Viruses · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Hepatitis C virus (HCV) often causes chronic infection and may lead to hepatocellular carcinoma (HCC). We have shown previously that HCV core protein has pleiotropic functions, including transcriptional regulation of a number of cellular genes, although the mechanism for gene regulation remains unclear. In this study, a mammalian two-hybrid screen identified a novel binding partner, HS1-associated protein X-1 (HAX-1), for HCV core protein from a human liver cDNA library. An association between HAX-1 and HCV core protein was further verified by confocal microscopy and coimmunoprecipitation in HepG2 cells expressing HCV core or full-length (FL) gene. Both HCV core protein and a chemotherapeutic agent for HCC, 5-flouorouracil (5-FU), are known to modulate p53. We examined here whether an association between core and HAX-1 has any functional relevance to p53 modulation in 5-FU-treated cells. For this, the role of HAX-1 on 5-FU treatment was examined in HepG2 cells expressing HCV core or FL gene using cell proliferation, p53 expression, and caspase activation analysis. Cells expressing HCV-core or FL gene were more susceptible to 5-FU-induced growth inhibition than control cells, whereas cell survival was enhanced after suppression of HAX-1 by small interfering RNA. Further, 5-FU-mediated p53 expression was reduced with concurrent HAX-1 suppression in core- or polyprotein-expressing cells compared to control HepG2 cells, and caspase-2 and -7 activities were diminished. On the other hand, HCV core protein did not play a detectable role in 5-FU-mediated caspase-7 activation in the absence of functional p53 in Hep3B or Huh-7 cells. These observations underscore an association between HCV core and HAX-1, which promotes 5-FU mediated p53-dependent caspase-7 activation and hepatocyte growth inhibition.
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Registered trials
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