ArticleThe Journal of biological chemistry2009
Mechanisms of activity-dependent plasticity in cellular nitric oxide-cGMP signaling.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Mathematical Model Predicts Effective Strategies to Inhibit VEGF-eNOS Signaling.Journal of clinical medicine · 2020Article
- Physiological activation and deactivation of soluble guanylate cyclase.Nitric oxide : biology and chemistry · 2018Review
- Mathematical Modelling of Nitric Oxide/Cyclic GMP/Cyclic AMP Signalling in Platelets.International journal of molecular sciences · 2018Article
- Erythrocytes do not activate purified and platelet soluble guanylate cyclases even in conditions favourable for NO synthesis.Cell communication and signaling : CCS · 2016Article
- Systems Pharmacology and Rational Polypharmacy: Nitric Oxide-Cyclic GMP Signaling Pathway as an Illustrative Example and Derivation of the General Case.PLoS computational biology · 2016Article
- From synaptically localized to volume transmission by nitric oxide.The Journal of physiology · 2016Review
- Motion of proximal histidine and structural allosteric transition in soluble guanylate cyclase.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Real-time monitoring the spatiotemporal dynamics of intracellular cGMP in vascular smooth muscle cells.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Improved genetically-encoded, FlincG-type fluorescent biosensors for neural cGMP imaging.Frontiers in molecular neuroscience · 2013Article
- Sub-Nanomolar Sensitivity of Nitric Oxide Mediated Regulation of cGMP and Vasomotor Reactivity in Vascular Smooth Muscle.Frontiers in pharmacology · 2012Article
- Dynamic ligand exchange in soluble guanylyl cyclase (sGC): implications for sGC regulation and desensitization.The Journal of biological chemistry · 2011Article
- Picomolar nitric oxide signals from central neurons recorded using ultrasensitive detector cells.The Journal of biological chemistry · 2011Article
- The ability of the skin to absorb heat; the effect of repeated exposure and age.Medical science monitor : international medical journal of experimental and clinical research · 2011Article
- Exquisite sensitivity to subsecond, picomolar nitric oxide transients conferred on cells by guanylyl cyclase-coupled receptors.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- New insight into the functioning of nitric oxide-receptive guanylyl cyclase: physiological and pharmacological implications.Molecular and cellular biochemistry · 2010Review
- REDUCTION IN SUBCUTANEOUS INSULIN REQUIREMENTS IN TETRAPLEGIC TYPE 1 DIABETIC WITH CERVICAL SPINAL CORD INJURY FOLLOWING PRAMLINTIDE TREATMENT.AACE clinical case reportsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cellular responsiveness to nitric oxide (NO) is shaped by past history of NO exposure. The mechanisms behind this plasticity were explored using rat platelets in vitro, specifically to determine the relative contributions made by desensitization of NO receptors, which couple to cGMP formation, and by phosphodiesterase-5 (PDE5), which is activated by cGMP and also hydrolyzes it. Repeated delivery of brief NO pulses (50 nM peak) at 1-min intervals resulted in a progressive loss of the associated cGMP responses, which was the combined consequence of receptor desensitization and PDE5 activation, with the former dominating. Delivery of pulses of differing amplitude showed that NO stimulated and desensitized receptors with similar potency (EC50 = 10-20 nM). PDE5 activation was highly sensitive to NO, with a single pulse peaking at 2 nM being sufficient to evoke a 50% loss of response to a subsequent near-maximal NO pulse. However, the activated state of the PDE subsided quickly after removal of NO, the half-time for recovery being 25 s. In contrast, receptor desensitization reverted much more slowly, the half-time being 16 min. Accordingly, with long (20-min) exposures, NO concentrations as low as 600 pM provoked significant desensitization. The results indicate that PDE5 activation and receptor desensitization subserve distinct short term and longer term roles as mediators of plasticity in NO-cGMP signaling. A kinetic model explicitly describing the complex interplay between NO concentration, cGMP synthesis, PDE5 activation, and the resulting cGMP accumulation successfully simulated the present and previous data.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.