ArticleBMC plant biology2009
A set of EST-SNPs for map saturation and cultivar identification in melon.
Article in BMC plant biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.
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Who cites it
52 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta genetic analysis of melon sweetness.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Pooled it
- Floral Characteristics and Reproductive Biology in Brazilian Melon Accessions: Insights from Commercial and Exotic Varieties.Plants (Basel, Switzerland) · 2025Article
- Polyphyletic domestication and inter-lineage hybridization magnified genetic diversity of cultivated melon,Breeding science · 2025Article
- Thorough Characterization ofFoods (Basel, Switzerland) · 2023Article
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- QTL Mapping for Gummy Stem Blight Resistance in Watermelon (Plants (Basel, Switzerland) · 2021Article
- QTLs and candidate genes analyses for fruit size under domestication and differentiation in melon (Cucumis melo L.) based on high resolution maps.BMC plant biology · 2021Article
- Comparative Metabolomics and Molecular Phylogenetics of Melon (Metabolites · 2020Article
- Re-evaluation of the role of Indian germplasm as center of melon diversification based on genotyping-by-sequencing analysis.BMC genomics · 2019Article
- A high-density linkage map and QTL mapping of fruit-related traits in pumpkin (Cucurbita moschata Duch.).Scientific reports · 2017Article
- Quantitative trait loci analysis of melon (Cucumis melo L.) domestication-related traits.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Genome-Wide Single Nucleotide Polymorphism Discovery and the Construction of a High-Density Genetic Map for Melon (Frontiers in plant science · 2017Article
- A high-density genetic map for anchoring genome sequences and identifying QTLs associated with dwarf vine in pumpkin (Cucurbita maxima Duch.).BMC genomics · 2015Article
- Construction of a high-density DArTseq SNP-based genetic map and identification of genomic regions with segregation distortion in a genetic population derived from a cross between feral and cultivated-type watermelon.Molecular genetics and genomics : MGG · 2015Article
- Article
- Article
- Identification of pummelo cultivars by using a panel of 25 selected SNPs and 12 DNA segments.PloS one · 2014Article
- Article
- Development of SSR markers by next-generation sequencing of Korean landraces of chamoe (Cucumis melo var. makuwa).Molecular biology reports · 2013Article
- Gold nanoparticle contrast agents in advanced X-ray imaging technologies.Molecules (Basel, Switzerland) · 2013Review
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13 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThere are few genomic tools available in melon (Cucumis melo L.), a member of the Cucurbitaceae, despite its importance as a crop. Among these tools, genetic maps have been constructed mainly using marker types such as simple sequence repeats (SSR), restriction fragment length polymorphisms (RFLP) and amplified fragment length polymorphisms (AFLP) in different mapping populations. There is a growing need for saturating the genetic map with single nucleotide polymorphisms (SNP), more amenable for high throughput analysis, especially if these markers are located in gene coding regions, to provide functional markers. Expressed sequence tags (ESTs) from melon are available in public databases, and resequencing ESTs or validating SNPs detected in silico are excellent ways to discover SNPs.
resultsEST-based SNPs were discovered after resequencing ESTs between the parental lines of the PI 161375 (SC) x 'Piel de sapo' (PS) genetic map or using in silico SNP information from EST databases. In total 200 EST-based SNPs were mapped in the melon genetic map using a bin-mapping strategy, increasing the map density to 2.35 cM/marker. A subset of 45 SNPs was used to study variation in a panel of 48 melon accessions covering a wide range of the genetic diversity of the species. SNP analysis correctly reflected the genetic relationships compared with other marker systems, being able to distinguish all the accessions and cultivars.
conclusionThis is the first example of a genetic map in a cucurbit species that includes a major set of SNP markers discovered using ESTs. The PI 161375 x 'Piel de sapo' melon genetic map has around 700 markers, of which more than 500 are gene-based markers (SNP, RFLP and SSR). This genetic map will be a central tool for the construction of the melon physical map, the step prior to sequencing the complete genome. Using the set of SNP markers, it was possible to define the genetic relationships within a collection of forty-eight melon accessions as efficiently as with SSR markers, and these markers may also be useful for cultivar identification in Occidental melon varieties.
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