ArticleMolecular genetics and genomics : MGG2009
Helix 69 in 23S rRNA modulates decoding by wild type and suppressor tRNAs.
Article in Molecular genetics and genomics : MGG, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 22 citations in OpenAlex.
- RNA sectors and allosteric function within the ribosome.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Endogenous rRNA Sequence Variation Can Regulate Stress Response Gene Expression and Phenotype.Cell reports · 2018Article
- Intersubunit Bridges of the Bacterial Ribosome.Journal of molecular biology · 2016Review
- Structure modulation of helix 69 from Escherichia coli 23S ribosomal RNA by pseudouridylations.Nucleic acids research · 2014Article
- Interplay of the bacterial ribosomal A-site, S12 protein mutations and paromomycin binding: a molecular dynamics study.PloS one · 2014Article
- The kissing-loop T-shaped structure translational enhancer of Pea enation mosaic virus can bind simultaneously to ribosomes and a 5' proximal hairpin.Journal of virology · 2013Article
- Role of pseudouridine in structural rearrangements of helix 69 during bacterial ribosome assembly.ACS chemical biology · 2012Article
- Comparison of solution conformations and stabilities of modified helix 69 rRNA analogs from bacteria and human.Biopolymers · 2012Article
- Helix 69 is key for uniformity during substrate selection on the ribosome.The Journal of biological chemistry · 2011Article
- Mutations in the intersubunit bridge regions of 16S rRNA affect decoding and subunit-subunit interactions on the 70S ribosome.Nucleic acids research · 2011Article
- Functional elucidation of a key contact between tRNA and the large ribosomal subunit rRNA during decoding.RNA (New York, N.Y.) · 2010Article
- Binding of aminoglycoside antibiotics to helix 69 of 23S rRNA.Nucleic acids research · 2010Article
- The structures of the anti-tuberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome.Nature structural & molecular biology · 2010Article
- Modulation of conformational changes in helix 69 mutants by pseudouridine modifications.Biophysical chemistryArticle
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Helix 69 of 23S rRNA forms one of the major inter-subunit bridges of the 70S ribosome and interacts with A- and P-site tRNAs and translation factors. Despite the proximity of h69 to the decoding center and tRNAs, the contribution of h69 to the tRNA selection process is unclear: previous genetic analyses have shown that h69 mutations increase frameshifting and readthrough of stop codons. However, a complete deletion of h69 does not affect the selection of cognate tRNAs in vitro. To address these discrepancies, the in vivo effects of a range of single- and multi-base h69 mutations in Escherichia coli 23S rRNA on various translation errors have been determined. While a majority of the h69 mutations examined here affected readthrough of stop codons and frameshifting, the DeltaA1916 single base deletion mutation uniquely influenced missense decoding. Different h69 mutants had either increased or decreased levels of stop codon readthrough. The h69 mutations that decreased UGA readthrough also decreased UGA reading by a mutant, near-cognate tRNA(Trp) carrying a G24A substitution in the D arm, but had far less effect on UGA reading by a suppressor tRNA with a complementary anticodon. These results suggest that h69 interactions with release factors contribute significantly to termination efficiency and that interaction with the D arm of A-site tRNA is important for discrimination between cognate and near-cognate tRNAs.
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