ArticleAnesthesiology2009
Depletion of calcium stores in injured sensory neurons: anatomic and functional correlates.
Article in Anesthesiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- PDX1, a transcription factor essential for organ differentiation, regulates SERCA-dependent CaCell calcium · 2024Article
- Dynamic changes in endoplasmic reticulum morphology and its contact with the plasma membrane in motor neurons in response to nerve injury.Cell and tissue research · 2024Article
- Sensory neuron dysfunction in orthotopic mouse models of colon cancer.Journal of neuroinflammation · 2022Article
- Restoration of Sarco/Endoplasmic Reticulum CaFrontiers in pharmacology · 2022Article
- Calcium in Neuronal and Glial Response to Axotomy.International journal of molecular sciences · 2021Review
- Genomic Action of Sigma-1 Receptor Chaperone Relates to Neuropathic Pain.Molecular neurobiology · 2021Article
- Article
- Parkinson's disease-associated LRRK2-G2019S mutant acts through regulation of SERCA activity to control ER stress in astrocytes.Acta neuropathologica communications · 2019Article
- From Mechanism to Cure: Renewing the Goal to Eliminate the Disease of Pain.Pain medicine (Malden, Mass.) · 2018Review
- Increased thrombospondin-4 after nerve injury mediates disruption of intracellular calcium signaling in primary sensory neurons.Neuropharmacology · 2017Article
- Painful nerve injury upregulates thrombospondin-4 expression in dorsal root ganglia.Journal of neuroscience research · 2015Article
- Divergent effects of painful nerve injury on mitochondrial Ca(2+) buffering in axotomized and adjacent sensory neurons.Brain research · 2014Article
- Painful nerve injury decreases sarco-endoplasmic reticulum Ca²⁺-ATPase activity in axotomized sensory neurons.Neuroscience · 2013Article
- Ca²⁺-dependent regulation of Ca²⁺ currents in rat primary afferent neurons: role of CaMKII and the effect of injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Oxytocin inhibits the membrane depolarization-induced increase in intracellular calcium in capsaicin sensitive sensory neurons: a peripheral mechanism of analgesic action.Anesthesia and analgesia · 2012Article
- Endoplasmic-reticulum calcium depletion and disease.Cold Spring Harbor perspectives in biology · 2011Review
- Store-operated Ca2+ entry in sensory neurons: functional role and the effect of painful nerve injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Time course of SERCA 2b and calreticulin expression in Purkinje neurons of ethanol-fed rats with behavioral correlates.Alcohol and alcoholism (Oxford, Oxfordshire)Article
- Labat lecture: the primary sensory neuron: where it is, what it does, and why it matters.Regional anesthesia and pain medicineArticle
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPainful nerve injury leads to disrupted Ca signaling in primary sensory neurons, including decreased endoplasmic reticulum (ER) Ca storage. This study examines potential causes and functional consequences of Ca store limitation after injury.
methodsNeurons were dissociated from axotomized fifth lumbar (L5) and the adjacent L4 dorsal root ganglia after L5 spinal nerve ligation that produced hyperalgesia, and they were compared to neurons from control animals. Intracellular Ca levels were measured with Fura-2 microfluorometry, and ER was labeled with probes or antibodies. Ultrastructural morphology was analyzed by electron microscopy of nondissociated dorsal root ganglia, and intracellular electrophysiological recordings were obtained from intact ganglia.
resultsLive neuron staining with BODIPY FL-X thapsigargin (Invitrogen, Carlsbad, CA) revealed a 40% decrease in sarco-endoplasmic reticulum Ca-ATPase binding in axotomized L5 neurons and a 34% decrease in L4 neurons. Immunocytochemical labeling for the ER Ca-binding protein calreticulin was unaffected by injury. Total length of ER profiles in electron micrographs was reduced by 53% in small axotomized L5 neurons, but it was increased in L4 neurons. Cisternal stacks of ER and aggregation of ribosomes occurred less frequently in axotomized neurons. Ca-induced Ca release, examined by microfluorometry with dantrolene, was eliminated in axotomized neurons. Pharmacologic blockade of Ca-induced Ca release with dantrolene produced hyperexcitability in control neurons, confirming its functional importance.
conclusionsAfter axotomy, ER Ca stores are reduced by anatomic loss and possibly diminished sarco-endoplasmic reticulum Ca-ATPase. The resulting disruption of Ca-induced Ca release and protein synthesis may contribute to the generation of neuropathic pain.
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