Evidence map›Paper›PMID 19602420›Full record

ArticleAnalytical biochemistry2009

Expression artifact with retroviral vectors based on pBMN.

Yoshitaka Fukunaga, Robert A Svoboda, Ronald L Cerny, Keith R Johnson, Margaret J Wheelock

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 84% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yoshitaka FukunagaDepartment of Oral Biology, University of Nebraska Medical Center, Omaha, 68198, USA.
Robert A Svoboda
Ronald L Cerny
Keith R Johnson
Margaret J Wheelock
University of Nebraska Medical Center · USUniversity of Nebraska–Lincoln · US

Funding

ASPECTS OF CADHERIN/CATENIN COMPLEXESR01GM051188 · NIGMS · UNIVERSITY OF TOLEDO · PI JOHNSON, KEITH R · 1994 to 2009
$3.4M
CADHERINS IN ORAL SQUAMOUS CELL CARCINOMASR01DE012308 · NIDCR · UNIVERSITY OF TOLEDO · PI JOHNSON, KEITH R · 1998 to 2007
$2.6M
Exploiting Novel Pathways to Treat Pancreatic CancerR21CA137401 · NCI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI JOHNSON, KEITH R · 2009 to 2010
$359k
NCI NIH HHS CA137401NCI NIH HHS R21 CA137401NIDCR NIH HHS DE12308NIDCR NIH HHS R01 DE012308NIGMS NIH HHS GM51188NIGMS NIH HHS R01 GM051188
6 · The paper itself

Abstract

While characterizing various splice forms of p120 catenin, we observed what appeared to be a novel posttranslational modification of p120, resulting in a higher molecular weight form that was dependent on the splicing pattern. Further investigation revealed the higher molecular weight form to be a fusion protein between sequences encoded by the retroviral vector and p120. We found that the publicly available sequence of the vector we used does not agree with the experimental sequence. We caution other investigators to be aware of this potential artifact.

Indexed as

ArtifactsExonsGene ExpressionTransfectionAlternative SplicingAmino Acid SequenceCateninsCell Adhesion MoleculesCell LineCodonDelta CateninDNA, ComplementaryGenetic VectorsHumansMolecular Sequence DataMoloney murine leukemia virusCateninsCell Adhesion MoleculesCodonDelta CateninDNA, ComplementaryPhosphoproteinsPolyproteinsProtein IsoformsRecombinant Fusion Proteins

Identifiers

PMID19602420
PMCPMC2753488
OpenAlexW2147446505

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.