ArticleBMC veterinary research2009
IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology.
Article in BMC veterinary research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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34 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- Beyond Serology: A Meta-Analysis of Advancements in Molecular Detection of Brucella spp. in Seronegative Animals and Biological Samples.Veterinary medicine and science · 2025Pooled it
- Brucellosis Abattoir Surveillance Using Serology and Molecular Tests Among Livestock in Free State and Limpopo Provinces, South Africa.Microorganisms · 2026Article
- Development of a high-copy target enhanced multiplexed crRNA-based, amplification-free detection assay (HiTECT) for Brucella spp.Molecular biology reports · 2026Article
- A novel loop-mediated isothermal amplification assay coupled with lateral flow assay for differential detection of Brucella spp from Brucella abortus S19 vaccine strain.Veterinary research communications · 2026Article
- Epidemiological baseline of Brucella spp. in South African wildlife.PLoS neglected tropical diseases · 2025Article
- Ultrasensitive and highly specific detection of theJournal of clinical microbiology · 2025Article
- Molecular and serological survey of Brucella spp. among rodents in western Iran.BMC veterinary research · 2025Article
- Unraveling brucellosis: advances in pathogenesis, diagnostic strategies, therapeutic innovations, and public health perspectives.Frontiers in medicine · 2025Review
- Article
- Bovine Brucellosis: First Comprehensive Evaluation from Hamedan, an Endemic Area in Iran.Indian journal of microbiology · 2024Article
- Epidemiological Investigation of Animal Brucellosis in Domestic Ruminants in Greece from 2015 to 2022 and Genetic Characterization of Prevalent Strains.Pathogens (Basel, Switzerland) · 2024Article
- Serological and molecular detection as well as typing ofHeliyon · 2024Article
- Seroprevalence and molecular detection of brucellosis among Pakistani women with spontaneous abortion.Frontiers in public health · 2024Article
- Evaluation of a Real-time PCR in Combination with a Cultivation Method for the Detection ofJournal of veterinary research · 2022Article
- Standardised Sampling Approach for Investigating Pathogens or Environmental Chemicals in Wild Game at Community Hunts.Animals : an open access journal from MDPI · 2022Article
- Ultrasensitive Detection of PathogenicFrontiers in veterinary science · 2022Article
- Development of a Genus-SpecificVeterinary sciences · 2020Article
- Monitoring results of wild boar (Infection ecology & epidemiology · 2020Article
- A Proof of Principle for the Detection of ViableMicroorganisms · 2020Article
- Optimization and validation of a real-time polymerase chain reaction protocol for the diagnosis of human brucellosis.Folia microbiologica · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundControl of brucellosis in livestock, wildlife and humans depends on the reliability of the methods used for detection and identification of bacteria. In the present study, we describe the evaluation of the recently established real-time PCR assay based on the Brucella-specific insertion sequence IS711 with blood samples from 199 wild boars (first group of animals) and tissue samples from 53 wild boars (second group of animals) collected in Switzerland. Results from IS711 real-time PCR were compared to those obtained by bacterial isolation, Rose Bengal Test (RBT), competitive ELISA (c-ELISA) and indirect ELISA (i-ELISA).
resultsIn the first group of animals, IS711 real-time PCR detected infection in 11.1% (16/144) of wild boars that were serologically negative. Serological tests showed different sensitivities [RBT 15.6%, c-ELISA 7.5% and i-ELISA 5.5%] and only 2% of blood samples were positive with all three tests, which makes interpretation of the serological results very difficult. Regarding the second group of animals, the IS711 real-time PCR detected infection in 26% of animals, while Brucella spp. could be isolated from tissues of only 9.4% of the animals.
conclusionThe results presented here indicate that IS711 real-time PCR assay is a specific and sensitive tool for detection of Brucella spp. infections in wild boars. For this reason, we propose the employment of IS711 real-time PCR as a complementary tool in brucellosis screening programs and for confirmation of diagnosis in doubtful cases.
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