ArticleAnalytical chemistry2009
Product length, dye choice, and detection chemistry in the bead-emulsion amplification of millions of single DNA molecules in parallel.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Exploring FGFR3 Mutations in the Male Germline: Implications for Clonal Germline Expansions and Paternal Age-Related Dysplasias.Genome biology and evolution · 2024Article
- Exploring the Micro-Mosaic Landscape ofGenes · 2024Article
- Artifactual mutations resulting from DNA lesions limit detection levels in ultrasensitive sequencing applications.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Aquaporin 5 expression in mouse mammary gland cells is not driven by promoter methylation.BioMed research international · 2015Article
- New evidence for positive selection helps explain the paternal age effect observed in achondroplasia.Human molecular genetics · 2013Article
- In vitro affinity screening of protein and peptide binders by megavalent bead surface display.Protein engineering, design & selection : PEDS · 2013Article
- Massively parallel haplotyping on microscopic beads for the high-throughput phase analysis of single molecules.PloS one · 2012Article
- Enhanced Single-Molecule Detection using Porous Silver Membrane.The journal of physical chemistry. C, Nanomaterials and interfaces · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The amplification of millions of single molecules in parallel can be performed on microscopic magnetic beads that are contained in aqueous compartments of an oil-buffer emulsion. These bead-emulsion amplification (BEA) reactions result in beads that are covered by almost-identical copies derived from a single template. The post-amplification analysis is performed using different fluorophore-labeled probes. We have identified BEA reaction conditions that efficiently produce longer amplicons of up to 450 base pairs. These conditions include the use of a Titanium Taq amplification system. Second, we explored alternate fluorophores coupled to probes for post-PCR DNA analysis. We demonstrate that four different Alexa fluorophores can be used simultaneously with extremely low crosstalk. Finally, we developed an allele-specific extension chemistry that is based on Alexa dyes to query individual nucleotides of the amplified material that is both highly efficient and specific.
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Registered trials
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