ArticleAnalytical chemistry2009
Gaussia luciferase variant for high-throughput functional screening applications.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 89 citations in OpenAlex.
- A Structural Model of Truncated Gaussia princeps Luciferase Elucidating the Crucial Catalytic Function of No.76 Arginine towards Coelenterazine Oxidation.PLoS computational biology · 2025Article
- Luciferase Reporter Systems in Investigating Interferon Antiviral Innate Immunity.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Development of a reporter feline herpesvirus-1 for antiviral screening assays.Veterinary research · 2024Article
- Advanced Bioluminescence Reporter with EngineeredBiosensors · 2024Article
- Molecular Dynamics Simulation Combined with Neural Relationship Inference and Markov Model to Reveal the Relationship between Conformational Regulation and Bioluminescence Properties ofMolecules (Basel, Switzerland) · 2024Article
- A high-throughputActa pharmaceutica Sinica. B · 2023Article
- Targeted Bioluminescent Imaging of Pancreatic Ductal Adenocarcinoma Using Nanocarrier-Complexed EGFR-Binding Affibody-Gaussia Luciferase Fusion Protein.Pharmaceutics · 2023Article
- Reflecting on mutational and biophysical analysis ofBiophysical reviews · 2022Review
- A Simple Secretion Assay for Assessing New and Existing Myocilin Variants.Current eye research · 2022Article
- Fluorescent and Luminescent Methods to Detect Lipolysis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Flash properties of Gaussia luciferase are the result of covalent inhibition after a limited number of cycles.Protein science : a publication of the Protein Society · 2021Article
- Solution structure of Gaussia Luciferase with five disulfide bonds and identification of a putative coelenterazine binding cavity by heteronuclear NMR.Scientific reports · 2020Article
- Gaussia Luciferase as a Reporter for Quorum Sensing inSensors (Basel, Switzerland) · 2020Article
- Rapid and sensitive large-scale screening of low affinity extracellular receptor protein interactions by using reaction induced inhibition of Gaussia luciferase.Scientific reports · 2020Article
- Methods for Establishing and Using a Stable Cell Line Expressing Both Gaussia Luciferase and Firefly Luciferase to Screen for Endoplasmic Reticulum Stress.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Genetically-encoded sensors to detect fatty acid production and trafficking.Molecular metabolism · 2019Article
- Review
- One-Step Ultrasensitive Bioluminescent Enzyme Immunoassay Based on Nanobody/Nanoluciferase Fusion for Detection of Aflatoxin BJournal of agricultural and food chemistry · 2019Article
- Bioluminescent reporter assay for monitoring ER stress in human beta cells.Scientific reports · 2018Article
- A High-Throughput Assay for Collagen Secretion Suggests an Unanticipated Role for Hsp90 in Collagen Production.Biochemistry · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Gaussia luciferase (Gluc) is a sensitive reporter for studying different biological processes such as gene expression, promoter activity, protein-protein interactions, signal transduction, as well as tumor cell growth and response to therapy. Since Gluc is naturally secreted, the kinetics of these processes can be monitored in real-time by measuring an aliquot of conditioned medium in culture or a few microliters of blood in vivo at different time points. Gluc catalyzes light emission with a short half-life which is unfavorable for certain applications. We isolated a Gluc mutant that catalyzes enhanced light stability in the presence of a detergent, in combination with high sensitivity, making it an attractive luciferase for high-throughput functional screening applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.