ArticleLangmuir : the ACS journal of surfaces and colloids2009
Direct biophotolithographic method for generating substrates with multiple overlapping biomolecular patterns and gradients.
Article in Langmuir : the ACS journal of surfaces and colloids, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Trial
- Article
- Quantitative developmental biology in vitro using micropatterning.Development (Cambridge, England) · 2021Review
- The combined effects of matrix stiffness and growth factor immobilization on the bioactivity and differentiation capabilities of adipose-derived stem cells.Biomaterials · 2014Article
- Strategies to balance covalent and non-covalent biomolecule attachment within collagen-GAG biomaterials.Biomaterials science · 2014Article
- Quantitative photochemical immobilization of biomolecules on planar and corrugated substrates: a versatile strategy for creating functional biointerfaces.ACS applied materials & interfaces · 2011Article
- Probing dynamic cell-substrate interactions using photochemically generated surface-immobilized gradients: application to selectin-mediated leukocyte rolling.Integrative biology : quantitative biosciences from nano to macro · 2011Article
- The generation of biomolecular patterns in highly porous collagen-GAG scaffolds using direct photolithography.Biomaterials · 2011Article
- Photolithography of Dithiocarbamate-Anchored Monolayers and Polymers on Gold.Journal of materials chemistry · 2011Article
- Research Spotlight: New multiparameter bioanalytical technologies for applications in personalized medicine, drug discovery and fundamental biology.Bioanalysis · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We describe an approach to generate multicomponent surface-immobilized patterns and gradients on the basis of the photochemically controlled covalent coupling of solution-phase biomolecules to benzophenone-modified substrates. Gradients were simply achieved by continuously varying the exposure to nondamaging UV light across the surface with the gradient profile controlled by biomolecule concentration and the spatial and temporal illumination of the surface. Sequential exposure of the same surface in the presence of different biomolecules resulted in overlapping patterns and gradients of proteins and carbohydrates. Finally, we preliminarily demonstrate that the resulting surfaces are suitable for generating model substrates to probe cell-substrate interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.