ArticleMolecular biology reports2010
Exogenous cMHM regulates the expression of DMRT1 and ER alpha in avian testes.
Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Medaka (Animals : an open access journal from MDPI · 2024Article
- Overview of Avian Sex Reversal.International journal of molecular sciences · 2023Review
- The m6A methylation regulates gonadal sex differentiation in chicken embryo.Journal of animal science and biotechnology · 2022Article
- Epigenetic regulation of male fate commitment from an initially bipotential system.Molecular and cellular endocrinology · 2018Review
- Article
- Masculine epigenetic sex marks of the CYP19A1/aromatase promoter in genetically male chicken embryonic gonads are resistant to estrogen-induced phenotypic sex conversion.Biology of reproduction · 2012Article
- Molecular analysis shows differential expression of R-spondin1 in zebrafish (Danio rerio) gonads.Molecular biology reports · 2011Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The male hypemethylated (MHM) region, associated with sex-specific DNA methylation and histone acetylation, encodes non-coding RNAs that are expressed exclusively on Z chromosome in female chickens. The function of the MHM non-coding RNAs is unclear yet. In order to analyze the regulation effect of MHM on mRNA expression of sex-dependent genes, exogenous plasmid containing chicken MHM (cMHM) was constructed and injected into left testes of 13-week-old roosters. Comb color of the roosters in the pEGFP-N1-cMHM group became much paler, an indication of change in sex hormones, as compared with that of the negative control groups at the 10 days after injection. Real-time PCR was used to determine mRNA expression of sex-dependent genes in testes tissue. Transcription of ER alpha gene was significantly up-regulated in the pEGFP-N1-cMHM group compared with that of the pEGFP-N1 and normal saline groups (P < 0.01), while expression of DMRT1 mRNA declined severely in the pEGFP-N1-cMHM group compared with that of normal saline groups (P < 0.05). Nevertheless, expression of other candidate genes, such as AMH, FOXL2, SOX9, and P450arom did not change after injection of exogenous cMHM. In conclusion, down-regulation of DMRT1 and up-regulation of ER alpha in rooster testes treated with exogenous pEGFP-N1-cMHM plasmid might indicate a novel cMHM non-coding RNA-mediated mechanism in avian sex differentiation.
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