ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2009
Development and assessment of simple PCR markers for SNP genotyping in barley.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 34 citations in OpenAlex.
- PCR Allele Competitive Extension (PACE) Workflow for Genotyping by SNP Identification.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A single nucleotide polymorphism in the "Fra" gene results in fractured starch granules in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Development and validation of KASP assays for genes underpinning key economic traits in bread wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Genome wide SNP discovery in flax through next generation sequencing of reduced representation libraries.BMC genomics · 2012Article
- SNP identification and allelic-specific PCR markers development for TaGW2, a gene linked to wheat kernel weight.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Molecular analysis of glycinin genes in soybean mutants for development of gene-specific markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Development of a temperature-switch PCR-based SNP typing method for Mycobacterium ulcerans.PLoS neglected tropical diseases · 2012Article
- Temperature switch PCR (TSP): Robust assay design for reliable amplification and genotyping of SNPs.BMC genomics · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simple molecular marker assays underpin routine plant breeding and research activities in many laboratories worldwide. With the rapid growth of single nucleotide polymorphism (SNP) resources for many important crop plants, the availability of routine, low-tech marker assays for genotyping SNPs is of increased importance. In this study, we demonstrate that temperature-switch PCR (TSP) supports the rapid development of robust, allele-specific PCR markers for codominant SNP genotyping on agarose gel. A total of 87 TSP markers for assessing gene diversity in barley were developed and used to investigate the efficacy for marker development, assay reliably and genotyping accuracy. The TSP markers described provide good coverage of the barley genome, are simple to use, easy to interpret and score, and are amenable to assay automation. They provide a resource of informative SNP markers for assessing genetic relationships among individuals, populations and gene pools of cultivated barley (Hordeum vulgare L.) and its wild relative H. spontaneum K. Koch. TSP markers provide opportunities to use available SNP resources for marker-assisted breeding and plant genetic research, and to generate information that can be integrated with SNP data from different sources and studies. TSP markers are expected to provide similar advantages for any animal or plant species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.