ArticleProceedings of the National Academy of Sciences of the United States of America2009
Quantification of nano-scale intermembrane contact areas by using fluorescence resonance energy transfer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 67 citations in OpenAlex.
- Exploration and analytical techniques for membrane curvature-sensing proteins in bacteria.Journal of bacteriology · 2025Review
- Coupling liquid phases in 3D condensates and 2D membranes: Successes, challenges, and tools.Biophysical journal · 2024Review
- Probing Activation and Conformational Dynamics of the Vesicle-Reconstituted βThe journal of physical chemistry. B · 2024Article
- Single Vesicle Fluorescence-Bleaching Assay for Multi-Parameter Analysis of Proteoliposomes by Total Internal Reflection Fluorescence Microscopy.ACS applied materials & interfaces · 2022Article
- Single-particle combinatorial multiplexed liposome fusion mediated by DNA.Nature chemistry · 2022Article
- How Membrane Geometry Regulates Protein Sorting Independently of Mean Curvature.ACS central science · 2020Article
- Single Proteoliposome High-Content Analysis Reveals Differences in the Homo-Oligomerization of GPCRs.Biophysical journal · 2018Article
- Membrane Curvature and Lipid Composition Synergize To Regulate N-Ras Anchor Recruitment.Biophysical journal · 2017Article
- Ultrasensitive Measurement of CaAngewandte Chemie (International ed. in English) · 2017Article
- Ratiometric Tension Probes for Mapping Receptor Forces and Clustering at Intermembrane Junctions.Nano letters · 2016Article
- Direct observation of proton pumping by a eukaryotic P-type ATPase.Science (New York, N.Y.) · 2016Article
- Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases.Nature chemical biology · 2015Article
- Coupled diffusion in lipid bilayers upon close approach.Journal of the American Chemical Society · 2015Article
- Shedding light on protein folding, structural and functional dynamics by single molecule studies.Molecules (Basel, Switzerland) · 2014Review
- Nanoscale high-content analysis using compositional heterogeneities of single proteoliposomes.Nature methods · 2014Article
- Geometrical membrane curvature as an allosteric regulator of membrane protein structure and function.Biophysical journal · 2014Article
- Membrane curvature sensing by amphipathic helices: a single liposome study using α-synuclein and annexin B12.The Journal of biological chemistry · 2011Article
- Intermembrane docking reactions are regulated by membrane curvature.Biophysical journal · 2011Article
- Mixing subattolitre volumes in a quantitative and highly parallel manner with soft matter nanofluidics.Nature nanotechnology · 2011Article
- Single vesicle assaying of SNARE-synaptotagmin-driven fusion reveals fast and slow modes of both docking and fusion and intrasample heterogeneity.Biophysical journal · 2011Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanometer-scale intermembrane contact areas (CAs) formed between single small unilamellar lipid vesicles (SUVs) and planar supported lipid bilayers are quantified by measuring fluorescence resonance energy transfer (FRET) between a homogenous layer of donor fluorophores labeling the supported bilayer and acceptor fluorophores labeling the SUVs. The smallest CAs detected in our setup between biotinylated SUVs and dense monolayers of streptavidin were approximately 20 nm in radius. Deformation of SUVs is revealed by comparing the quenching of the donors to calculations of FRET between a perfectly spherical shell and a flat surface containing complementary fluorophores. These results confirmed the theoretical prediction that the degree of deformation scales with the SUV diameter. The size of the CA can be controlled experimentally by conjugating polyethylene glycol polymers to the SUV or the surface and thereby modulating the interfacial energy of adhesion. In this manner, we could achieve secure immobilization of SUVs under conditions of minimal deformation. Finally, we demonstrate that kinetic measurements of CA, at constant adhesion, can be used to record in real-time quantitative changes in the bilayer tension of a nano-scale lipid membrane system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.