ArticleGenetics2009
Temperature-sensitive mutations made easy: generating conditional mutations by using temperature-sensitive inteins that function within different temperature ranges.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Development of a novel simplified Q-system with integrated temporal expression control.bioRxiv : the preprint server for biology · 2026Article
- Approaches to Study Proteins Encoded by Essential Genes.Proteins · 2026Review
- Intein-based thermoregulated meganucleases for containment of genetic material.Nucleic acids research · 2024Article
- Applying the auxin-based degron system for the inducible, reversible and complete protein degradation iniScience · 2022Article
- Gene drive that results in addiction to a temperature-sensitive version of an essential gene triggers population collapse inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster.PLoS genetics · 2021Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- Use of Machine Learning with Temporal Photoluminescence Signals from CdTe Quantum Dots for Temperature Measurement in Microfluidic Devices.ACS applied nano materials · 2020Article
- Complex modifier landscape underlying genetic background effects.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Rational elicitation of cold-sensitive phenotypes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Members of the RAD52 Epistasis Group Contribute to Mitochondrial Homologous Recombination and Double-Strand Break Repair in Saccharomyces cerevisiae.PLoS genetics · 2015Article
- Temperature-sensitive splicing of mitfa by an intron mutation in zebrafish.Pigment cell & melanoma research · 2015Article
- Dynamics differentiate between active and inactive inteins.European journal of medicinal chemistry · 2015Article
- How to understand and outwit adaptation.Developmental cell · 2014Article
- Article
- Internal disulfide bond acts as a switch for intein activity.Biochemistry · 2013Article
- Recent progress in intein research: from mechanism to directed evolution and applications.Cellular and molecular life sciences : CMLS · 2013Review
- Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources.The Journal of biological chemistry · 2012Article
- An intein with genetically selectable markers provides a new approach to internally label proteins with GFP.BMC biotechnology · 2011Article
- Biomolecular engineering of intracellular switches in eukaryotes.Journal of drug delivery science and technology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reversible and easy to use, temperature-sensitive (TS) mutations are powerful tools for studying gene function. However, TS alleles are rare and difficult to generate and identify, and this has limited their use in most multicellular organisms. We have generated and characterized 41 intein switches, temperature-sensitive Sce VMA mutations that splice only at the permissive temperatures to generate intact host proteins. At nonpermissive temperatures, they fail to splice, resulting in a loss of function of the proteins in which they reside. By inserting an intein switch into a protein of interest, one can turn on and off the activities of the engineered protein with a simple temperature shift. The 41 TS inteins function in five different temperature ranges, with permissive temperatures ranging from 18 degrees to 30 degrees . This collection makes it possible to choose a TS-intein switch according to the optimal growth temperature of an organism or to suit a special experimental design.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.