ArticleJournal of cell science2009
Tssk6 is required for Izumo relocalization and gamete fusion in the mouse.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed, 1 synthesis or guideline pooled it, 158 citations in OpenAlex.
- The Role of Sperm Proteins IZUMO1 and TMEM95 in Mammalian Fertilization: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Doublet microtubule-associated tektins and enzymes differentially regulate sperm flagellar integrity and motility.Nature communications · 2026Article
- Efficacy and safety evaluation of HyperSperm treatment in human semen samples.Journal of translational medicine · 2026Article
- Membrane Fusions During Mammalian Fertilization.Advances in experimental medicine and biology · 2026Review
- A single-cell multiomics roadmap of zebrafish spermatogenesis reveals regulatory principles of male germline formation.Molecular systems biology · 2026Article
- TSSKL is essential for sperm mitochondrial morphogenesis and male fertility in moths.PLoS genetics · 2025Article
- Identification of TSSK1 and TSSK2 as Novel Targets for Male Contraception.Biomolecules · 2025Article
- Assessing Bovine Male Fertility in a Technological Age.Advances in anatomy, embryology, and cell biology · 2025Review
- Characterization of sexual maturity-associated N6-methyladenosine in boar testes.BMC genomics · 2024Article
- IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa.Biomedicines · 2023Article
- Expression of Testis-specific Serine/Threonine Kinases during the Reproductive and Nonreproductive Seasons and Their Localization in Mature Spermatozoa of Tree Shrews (Comparative medicine · 2023Article
- Article
- Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa.International journal of biological sciences · 2023Article
- Comparative analysis of the testes from wild-type and Alkbh5-knockout mice using single-cell RNA sequencing.G3 (Bethesda, Md.) · 2022Article
- Mysteries and unsolved problems of mammalian fertilization and related topics.Biology of reproduction · 2022Review
- Sperm proteins ODF2 and PAWP as markers of fertility in breeding bulls.Cell and tissue research · 2022Article
- High Resolution Proteomic Analysis of Subcellular Fractionated Boar Spermatozoa Provides Comprehensive Insights Into Perinuclear Theca-Residing Proteins.Frontiers in cell and developmental biology · 2022Article
- TSSK3, a novel target for male contraception, is required for spermiogenesis.Molecular reproduction and development · 2021Article
- Cyclic FEE Peptide Improves Human Sperm Movement Parameters without Modification of Their Energy Metabolism.International journal of molecular sciences · 2021Article
- The cell biology of fertilization: Gamete attachment and fusion.The Journal of cell biology · 2021Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
One of the most important processes in fertilization is the fusion of egg and sperm; however, the molecular mechanisms involved in this process are not well understood. So far, using genetic approaches, only two proteins have been demonstrated to be necessary for this process: Izumo in sperm and CD9 in the egg. Here we demonstrate that sperm produced by Tssk6 (Sstk)-null mice present defects that prevent the successful fertilization of eggs in vitro and the fusion to zona-pellucida-free eggs. Tssk6 is a member of the testis-specific serine kinase family of proteins and is expressed postmeiotically in male germ cells. In order for fusion to occur, during the process known as acrosome reaction Izumo needs to relocate from the anterior head to other regions, including the postacrosomal compartment. Tssk6-null sperm fails to relocate Izumo during the acrosome reaction. Agents that interfere with actin dynamics blocked the acrosome-reaction-associated translocation of Izumo that is required for fusion in wild-type sperm. Additionally, actin polymerization was compromised in Tssk6-null sperm. Taken together, our results indicate that Tssk6 is involved in sperm-egg fusion through the regulation of actin polymerization and changes in Izumo localization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.