ArticleAnalytical biochemistry2009
Fully automated protein purification.
Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- REVOLVER: A low-cost automated protein purifier based on parallel preparative gravity column workflows.HardwareX · 2022Article
- A mechanistic examination of salting out in protein-polymer membrane interactions.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Molecular modification, expression and purification of new subtype antioxidant peptide fromJournal of food science and technology · 2018Article
- Multi-position data collection and dynamic beam sizing: recent improvements to the automatic data-collection algorithms on MASSIF-1.Acta crystallographica. Section D, Structural biology · 2018Article
- Enabling Droplet Functionality on Anisotropic Ratchet Conveyors.Micromachines · 2017Article
- Different Stationary Phase Selectivities and Morphologies for Intact Protein Separations.Chromatographia · 2017Review
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- Article
- Automated hydrophobic interaction chromatography column selection for use in protein purification.Journal of visualized experiments : JoVE · 2011Article
- To automate or not to automate: this is the question.Journal of structural and functional genomics · 2010Article
- Building protein networks in synthetic systems from the bottom-up.Biotechnology advancesReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Obtaining highly purified proteins is essential to begin investigating their functional and structural properties. The steps that are typically involved in purifying proteins can include an initial capture, intermediate purification, and a final polishing step. Completing these steps can take several days and require frequent attention to ensure success. Our goal was to design automated protocols that would allow the purification of proteins with minimal operator intervention. Separate methods have been produced and tested that automate the sample loading, column washing, sample elution and peak collection steps for ion exchange, metal affinity, hydrophobic interaction, and gel filtration chromatography. These individual methods are designed to be coupled and run sequentially in any order to achieve a flexible and fully automated protein purification protocol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.