ArticleBMC genomics2009
Gene expression responses in male fathead minnows exposed to binary mixtures of an estrogen and antiestrogen.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 83 citations in OpenAlex.
- Characterization of vitellogenin concentration in male fathead minnow mucus compared to plasma, and liver mRNA.Ecotoxicology and environmental safety · 2022Article
- Reproductive health and endocrine disruption in smallmouth bass (Micropterus dolomieu) from the Lake Erie drainage, Pennsylvania, USA.Environmental monitoring and assessment · 2021Article
- Cross-species physiological interactions of endocrine disrupting chemicals with the circadian clock.General and comparative endocrinology · 2021Review
- Downregulation of testosterone production through luteinizing hormone receptor regulation in male rats exposed to 17α-ethynylestradiol.Scientific reports · 2020Article
- Twenty years of transcriptomics, 17alpha-ethinylestradiol, and fish.General and comparative endocrinology · 2020Review
- Tissue-Based Mapping of the Fathead Minnow (Frontiers in endocrinology · 2018Article
- Persistent Effects of Developmental Exposure to 17α-Ethinylestradiol on the Zebrafish (Frontiers in behavioral neuroscience · 2017Article
- Dietary exposure of 17-alpha ethinylestradiol modulates physiological endpoints and gene signaling pathways in female largemouth bass (Micropterus salmoides).Aquatic toxicology (Amsterdam, Netherlands) · 2014Article
- The sex-specific transcriptome of the hermaphrodite sparid sharpsnout seabream (Diplodus puntazzo).BMC genomics · 2014Article
- Exploring androgen-regulated pathways in teleost fish using transcriptomics and proteomics.Integrative and comparative biology · 2012Review
- Development of a transient expression assay for detecting environmental oestrogens in zebrafish and medaka embryos.BMC biotechnology · 2012Article
- Affinity and matrix effects in measuring fish plasma vitellogenin using immunosorbent assays: considerations for aquatic toxicologists.ISRN toxicology · 2012Article
- Effect-based tools for monitoring and predicting the ecotoxicological effects of chemicals in the aquatic environment.Sensors (Basel, Switzerland) · 2012Review
- Applications for next-generation sequencing in fish ecotoxicogenomics.Frontiers in genetics · 2012Article
- Transcriptomics responses in marine diatom Thalassiosira pseudonana exposed to the polycyclic aromatic hydrocarbon benzo[a]pyrene.PloS one · 2011Article
- Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques.BMC genomics · 2010Article
- Site-specific impacts on gene expression and behavior in fathead minnows (Pimephales promelas) exposed in situ to streams adjacent to sewage treatment plants.BMC bioinformatics · 2009Article
Corrections and comments
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Authors and funding
10 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAquatic organisms are continuously exposed to complex mixtures of chemicals, many of which can interfere with their endocrine system, resulting in impaired reproduction, development or survival, among others. In order to analyze the effects and mechanisms of action of estrogen/anti-estrogen mixtures, we exposed male fathead minnows (Pimephales promelas) for 48 hours via the water to 2, 5, 10, and 50 ng 17alpha-ethinylestradiol (EE2)/L, 100 ng ZM 189,154/L (a potent antiestrogen known to block activity of estrogen receptors) or mixtures of 5 or 50 ng EE(2)/L with 100 ng ZM 189,154/L. We analyzed gene expression changes in the gonad, as well as hormone and vitellogenin plasma levels.
resultsSteroidogenesis was down-regulated by EE(2) as reflected by the reduced plasma levels of testosterone in the exposed fish and down-regulation of genes in the steroidogenic pathway. Microarray analysis of testis of fathead minnows treated with 5 ng EE(2)/L or with the mixture of 5 ng EE(2)/L and 100 ng ZM 189,154/L indicated that some of the genes whose expression was changed by EE(2) were blocked by ZM 189,154, while others were either not blocked or enhanced by the mixture, generating two distinct expression patterns. Gene ontology and pathway analysis programs were used to determine categories of genes for each expression pattern.
conclusionOur results suggest that response to estrogens occurs via multiple mechanisms, including canonical binding to soluble estrogen receptors, membrane estrogen receptors, and other mechanisms that are not blocked by pure antiestrogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.