ArticleStem cells (Dayton, Ohio)2009
Importin 13 serves as a potential marker for corneal epithelial progenitor cells.
Article in Stem cells (Dayton, Ohio), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Hyaluronan supports the limbal stem cell phenotype during ex vivo culture.Stem cell research & therapy · 2022Article
- Importin 13 promotes NSCLC progression by mediating RFPL3 nuclear translocation and hTERT expression upregulation.Cell death & disease · 2020Article
- Role of Nucleoporins and Transport Receptors in Cell Differentiation.Frontiers in physiology · 2020Review
- Karyopherins in cancer.Current opinion in cell biology · 2018Review
- Poly(ethylene glycol)-modified silk fibroin membrane as a carrier for limbal epithelial stem cell transplantation in a rabbit LSCD model.Stem cell research & therapy · 2017Article
- Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells.International journal of ophthalmology · 2017Article
- ROS-mediated Different Homeostasis of Murine Corneal Epithelial Progenitor Cell Line under Oxidative Stress.Scientific reports · 2016Article
- A novel method in preparation of acellularporcine corneal stroma tissue for lamellar keratoplasty.American journal of translational research · 2015Article
- Expression and subcellular distribution of imp13 are regulated in brain development.In vitro cellular & developmental biology. Animal · 2013Article
- Increased importin 13 activity is associated with the pathogenesis of pterygium.Molecular vision · 2013Article
- Atomic force microscopy analysis of progenitor corneal epithelial cells fractionated by a rapid centrifugation isolation technique.PloS one · 2013Article
- Comparison of the transcriptomes of long-term label retaining-cells and control cells microdissected from mammary epithelium: an initial study to characterize potential stem/progenitor cells.Frontiers in oncology · 2013Article
- ES micro-environment enhances stemness and inhibits apoptosis in human limbal stem cells via the maintenance of telomerase activity.PloS one · 2013Article
- Increased expression of importin13 in endometriosis and endometrial carcinoma.Medical science monitor : international medical journal of experimental and clinical research · 2012Article
- Evaluation of novel decellularizing corneal stroma for cornea tissue engineering applications.International journal of ophthalmology · 2012Article
- Karyopherins in nuclear transport of homeodomain proteins during development.Biochimica et biophysica acta · 2011Review
- The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps.Medical science monitor : international medical journal of experimental and clinical research · 2011Article
- Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix.In vitro cellular & developmental biology. Animal · 2010Article
Corrections and comments
- Erratum issued
Authors and funding
13 authors at 4 institutions in 3 countries.
Funding
Abstract
Importin13 (IPO13), the newest member of importin-beta family discovered recently, is a unique nucleus-cytoplasm bidirectional transport receptor protein. In this study, IPO13 expression in human corneal tissue, limbal epithelial primary explant and clonal culture was evaluated by immunostaining and reverse-transcription polymerase chain reasgon. IPO13 function was evaluated in the corneal epithelial culture treated with IPO13 inhibitor, or fetal bovine serum (FBS)-containing Dulbecco's modified Eagle's medium (DMEM) medium by colony-forming efficiency, clone growth capacity, MTT, immunostaining, and Western blotting assay. IPO13 protein was expressed mainly in nuclei of limbal epithelial basal cells, but not in the other cell layers of limbus and full thickness of corneal epithelia. IPO13 was expressed in the majority of epithelial cells in early-stage clones and in the margin of late-stage clones. IPO13 was positively expressed in mouse TKE2 progenitor cells cultured in keratinocyte serum-free defined medium, while it became negative in FBS-containing DMEM, which promoted TKE2 cell differentiation. In the presence of IPO13 inhibitor, IPO13 expression and the proliferative capacity decreased in human limbal epithelial clones and mouse TKE2 cells, which were accompanied with the cell differentiation. In conclusion, our findings demonstrate for the first time that IPO13 is uniquely expressed by human limbal basal epithelial cells, and plays an important role in maintaining the phenotype, high proliferative potential, and less differentiation of corneal epithelial progenitor cells, suggesting that IPO13 could serve as a novel potential marker for corneal epithelial progenitor cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.