ArticlePloS one2009
Base flipping in tn10 transposition: an active flip and capture mechanism.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The telomere resolvase, TelA, utilizes an underwound pre-cleavage intermediate to promote hairpin telomere formation.PloS one · 2023Article
- Jumping Ahead withViruses · 2021Review
- A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming.Nucleic acids research · 2020Article
- A single active site in the mariner transposase cleaves DNA strands of opposite polarity.Nucleic acids research · 2017Article
- Sequence dependency of canonical base pair opening in the DNA double helix.PLoS computational biology · 2017Article
- Article
- Modern aspects of the structural and functional organization of the DNA mismatch repair system.Acta naturae · 2013Article
- An enzyme-catalyzed multistep DNA refolding mechanism in hairpin telomere formation.PLoS biology · 2013Article
- H-NS mediates the dissociation of a refractory protein-DNA complex during Tn10/IS10 transposition.Nucleic acids research · 2011Article
- A simple topological filter in a eukaryotic transposon as a mechanism to suppress genome instability.Molecular and cellular biology · 2011Article
- Use of nucleic Acid analogs for the study of nucleic Acid interactions.Journal of nucleic acids · 2011Article
- Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequences.Genetica · 2010Review
- Review
- Integrating prokaryotes and eukaryotes: DNA transposases in light of structure.Critical reviews in biochemistry and molecular biology · 2010Review
- Transposition of the human Hsmar1 transposon: rate-limiting steps and the importance of the flanking TA dinucleotide in second strand cleavage.Nucleic acids research · 2010Article
- Base flipping in V(D)J recombination: insights into the mechanism of hairpin formation, the 12/23 rule, and the coordination of double-strand breaks.Molecular and cellular biology · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The bacterial Tn5 and Tn10 transposases have a single active site that cuts both strands of DNA at their respective transposon ends. This is achieved using a hairpin intermediate that requires the DNA to change conformation during the reaction. In Tn5 these changes are controlled in part by a flipped nucleoside that is stacked on a tryptophan residue in a hydrophobic pocket of the transposase. Here we have investigated the base flipping mechanism in Tn10 transposition. As in Tn5 transposition, we find that base flipping takes place after the first nick and is required for efficient hairpin formation and resolution. Experiments with an abasic substrate show that the role of base flipping in hairpin formation is to remove the base from the DNA helix. Specific interactions between the flipped base and the stacking tryptophan residue are required for hairpin resolution later in the reaction. We show that base flipping in Tn10 transposition is not a passive reaction in which a spontaneously flipped base is captured and retained by the protein. Rather, it is driven in part by a methionine probe residue that helps to force the flipped base from the base stack. Overall, it appears that base flipping in Tn10 transposition is similar to that in Tn5 transposition.
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