ArticleJournal of immunology (Baltimore, Md. : 1950)2009
Palmitoylation-dependent plasma membrane transport but lipid raft-independent signaling by linker for activation of T cells.
Article in Journal of immunology (Baltimore, Md. : 1950), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 54 citations in OpenAlex.
- Kv1.3 palmitoylation regulates spatial distribution and channel removal from the immunological synapse.Cellular and molecular life sciences : CMLS · 2026Article
- Palmitoylation-dependent regulation of innate and adaptive immunity: molecular insights and translational opportunities.Cellular & molecular biology letters · 2026Review
- A novel prognostic model for ovarian cancer: construction, validation, and therapeutic insights.Frontiers in genetics · 2026Article
- Protein lipidation in the tumor microenvironment: enzymology, signaling pathways, and therapeutics.Molecular cancer · 2025Review
- Step-Wise Assembly of LAT Signaling Clusters Immediately After T Cell Receptor Triggering Contributes to Signal Propagation.International journal of molecular sciences · 2025Article
- Redox Regulation of LAT Enhances T Cell-Mediated Inflammation.Antioxidants (Basel, Switzerland) · 2024Article
- The anti-inflammatory role of zDHHC23 through the promotion of macrophage M2 polarization and macrophage necroptosis in large yellow croaker (Frontiers in immunology · 2024Article
- Identification of a New Cholesterol-Binding Site within the IFN-γ Receptor that is Required for Signal Transduction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- S-acylation of Orai1 regulates store-operated Ca2+ entry.Journal of cell science · 2022Article
- Protein cysteine palmitoylation in immunity and inflammation.The FEBS journal · 2021Review
- Detection of Protein S-Acylation using Acyl-Resin Assisted Capture.Journal of visualized experiments : JoVE · 2020Article
- Acylation - A New Means to Control Traffic Through the Golgi.Frontiers in cell and developmental biology · 2019Review
- Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.The Journal of experimental medicine · 2018Article
- Progress toward Understanding Protein S-acylation: Prospective in Plants.Frontiers in plant science · 2017Review
- Tetraspanins and Transmembrane Adaptor Proteins As Plasma Membrane Organizers-Mast Cell Case.Frontiers in cell and developmental biology · 2016Review
- Modulation of cell surface transport and lipid raft localization by the cytoplasmic tail of the influenza virus hemagglutinin.Cellular microbiology · 2016Article
- The linker for activation of T cells (LAT) signaling hub: from signaling complexes to microclusters.The Journal of biological chemistry · 2015Review
- Rapid and transient palmitoylation of the tyrosine kinase Lck mediates Fas signaling.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- C-type lectin like receptor 2 (CLEC-2) signals independently of lipid raft microdomains in platelets.Biochemical pharmacology · 2015Article
- Membrane raft association is a determinant of plasma membrane localization.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Linker for activation of T cells (LAT) is a dually palmitoylated transmembrane adaptor protein essential for T cell development and activation. However, whether LAT palmitoylation and/or lipid raft localization are required for its function is controversial. To address this question, we used a combination of biochemical, imaging, and genetic approaches, including LAT retrovirus-transduced mouse T cells and bone marrow chimeric mice. A nonpalmitoylated, non-lipid raft-residing mutant of transmembrane LAT could not reconstitute T cell development in bone marrow chimeric mice. This mutant was absent from the plasma membrane (PM) and was restricted mainly to the Golgi apparatus. A chimeric, nonpalmitoylated LAT protein consisting of the PM-targeting N-terminal sequence of Src kinase and the LAT cytoplasmic domain (Src-LAT) localized as a peripheral membrane protein in the PM, but outside lipid rafts. Nevertheless, Src-LAT restored T cell development and activation. Lastly, monopalmitoylation of LAT on Cys(26) (but not Cys(29)) was required and sufficient for its PM transport and function. Thus, the function of LAT in T cells requires its PM, but not raft, localization, even when expressed as a peripheral membrane protein. Furthermore, LAT palmitoylation functions primarily as a sorting signal required for its PM transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.