ArticleApplied and environmental microbiology2009
Quantitative PCR for determining the infectivity of bacteriophage MS2 upon inactivation by heat, UV-B radiation, and singlet oxygen: advantages and limitations of an enzymatic treatment to reduce false-positive results.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 1 of them a synthesis that pooled it.
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Who cites it
57 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?FEMS microbiology reviews · 2023Pooled it
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- Elimination of Airborne Microorganisms Using Compressive Heating Air Sterilization Technology (CHAST): Laboratory and Nursing Home Setting.Microorganisms · 2025Article
- Benchmarking Concentration and Extraction Methods for Wastewater-Based Surveillance of Eight Human Respiratory Viruses: Implications for Rapid Application to Novel Pathogens.Environmental science & technology · 2025Article
- Utilization of semiconductor-decorated tungsten trioxide nanowire membranes for the elimination of microorganisms from potable water.RSC advances · 2025Article
- Zooplankton protect viruses from sunlight disinfection.Applied and environmental microbiology · 2025Article
- Chemical and Heat Treatment for Viral Inactivation in Porcine-Derived Gelatin.BMC biotechnology · 2024Article
- Heat inactivation of aqueous viable norovirus and MS2 bacteriophage.Journal of applied microbiology · 2024Article
- The Protective Effect of Virus Capsids on RNA and DNA Virus Genomes in Wastewater.Environmental science & technology · 2023Article
- Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on HumanMaterials (Basel, Switzerland) · 2022Article
- Optimizing Human Intestinal Enteroids for Environmental Monitoring of Human Norovirus.Food and environmental virology · 2021Article
- A direct capture method for purification and detection of viral nucleic acid enables epidemiological surveillance of SARS-CoV-2.The Science of the total environment · 2021Article
- Monitoring waves of the COVID-19 pandemic: Inferences from WWTPs of different sizes.The Science of the total environment · 2021Article
- Decay of infectious SARS-CoV-2 and surrogates in aquatic environments.Water research · 2021Article
- Evaluation of two rapid ultrafiltration-based methods for SARS-CoV-2 concentration from wastewater.The Science of the total environment · 2021Article
- Review
- Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S.Environmental science : water research & technology · 2021Article
- Article
- Humidity and Deposition Solution Play a Critical Role in Virus Inactivation by Heat Treatment of N95 Respirators.mSphere · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Health risks posed by waterborne viruses are difficult to assess because it is tedious or impossible to determine the infectivity of many viruses. Recent studies hypothesized that quantitative PCR (qPCR) could selectively quantify infective viruses if preceded by an enzymatic treatment (ET) to reduce confounding false-positive signals. The goal of this study was to determine if ET with qPCR (ET-qPCR) can be used to accurately quantify the infectivity of the human viral surrogate bacteriophage MS2 upon partial inactivation by three treatments (heating at 72 degrees C, singlet oxygen, and UV radiation). Viruses were inactivated in buffered solutions and a lake water sample and assayed with culturing, qPCR, and ET-qPCR. To ensure that inactivating genome damage was fully captured, primer sets that covered the entire coding region were used. The susceptibility of different genome regions and the maximum genomic damage after each inactivating treatment were compared. We found that (i) qPCR alone caused false-positive results for all treatments, (ii) ET-qPCR significantly reduced (up to >5.2 log units) but did not eliminate the false-positive signals, and (iii) the elimination of false-positive signals differed between inactivating treatments. By assaying the whole coding region, we demonstrated that genome damage only partially accounts for virus inactivation. The possibility of achieving complete accordance between culture- and PCR-based assays is therefore called into doubt. Despite these differences, we postulate that ET-qPCR can track infectivity, given that decreases in infectivity were always accompanied by dose-dependent decreases in ET-qPCR signal. By decreasing false-positive signals, ET-qPCR improved the detection of infectivity loss relative to qPCR.
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