ArticleApplied and environmental microbiology2009
Quantitative PCR for genetic markers of human fecal pollution.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Effect of Sanitation Improvements on Pathogens and Microbial Source Tracking Markers in the Rural Bangladeshi Household Environment.Environmental science & technology · 2020Trial
- Microbial source tracking of human and animal fecal contamination in Ecuadorian households.Applied and environmental microbiology · 2026Article
- Novel Bacteroides bacteriophage-like quantitative real-time PCR assays for human sewage pollution measurement.Environmental pollution (Barking, Essex : 1987) · 2025Article
- Microbial source tracking of human and animal fecal contamination in Ecuadorian households.bioRxiv : the preprint server for biology · 2025Article
- An integrated approach to coupled nutrient and microbial source tracking in an agricultural watershed.Water research · 2025Article
- Application of a microbial and pathogen source tracking toolbox to identify infrastructure problems in stormwater drainage networks: a case study.Microbiology spectrum · 2024Article
- Quantitative fecal source characterization of urban municipal storm sewer system outfall 'wet' and 'dry' weather discharges.Water research · 2024Article
- Occurrence, Sources and Virulence Potential ofEnvironmental science & technology · 2024Article
- Occurrence of recreational water quality monitoring general fecal indicator bacteria and fecal source identification genetic markers in gray seal scat.The Science of the total environment · 2024Article
- Article
- Genetic fecal source identification in urban streams impacted by municipal separate storm sewer system discharges.PloS one · 2023Article
- Interlaboratory performance and quantitative PCR data acceptance metrics for NIST SRM® 2917.Water research · 2022Article
- Performance of NIST SRM® 2917 with 13 recreational water quality monitoring qPCR assays.Water research · 2022Article
- Technical framework for wastewater-based epidemiology of SARS-CoV-2.The Science of the total environment · 2021Article
- Aminoglycosides Antagonize Bacteriophage Proliferation, Attenuating Phage Suppression of Bacterial Growth, Biofilm Formation, and Antibiotic Resistance.Applied and environmental microbiology · 2021Article
- Variable fecal source prioritization in recreational waters routinely monitored with viral and bacterial general indicators.Water research · 2021Article
- Bather Shedding as a Source of Human Fecal Markers to a Recreational Beach.Frontiers in microbiology · 2021Article
- CrAssphage for fecal source tracking in Chile: Covariation with norovirus, HF183, and bacterial indicators.Water research X · 2020Article
- Contamination Scenario Matters when Using Viral and Bacterial Human-Associated Genetic Markers as Indicators of a Health Risk in Untreated Sewage-Impacted Recreational Waters.Environmental science & technology · 2020Article
- Fecal pollution source characterization at non-point source impacted beaches under dry and wet weather conditions.Water research · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Assessment of health risk and fecal bacterial loads associated with human fecal pollution requires reliable host-specific analytical methods and a rapid quantification approach. We report the development of quantitative PCR assays for quantification of two recently described human-specific genetic markers targeting Bacteroidales-like cell surface-associated genes. Each assay exhibited a range of quantification from 10 to 1 x 10(6) copies of target DNA. For each assay, internal amplification controls were developed to detect the presence or absence of amplification inhibitors. The assays predominantly detected human fecal specimens and exhibited specificity levels greater than 97% when tested against 265 fecal DNA extracts from 22 different animal species. The abundance of each human-specific genetic marker in primary effluent wastewater samples collected from 20 geographically distinct locations was measured and compared to quantities estimated by real-time PCR assays specific for rRNA gene sequences from total Bacteroidales and enterococcal fecal microorganisms. Assay performances combined with the prevalence of DNA targets in sewage samples provide experimental evidence supporting the potential application of these quantitative methods for monitoring fecal pollution in ambient environmental waters.
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