ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2009
In situ reverse-transcription loop-mediated isothermal amplification (in situ RT-LAMP) for detection of Japanese encephalitis viral RNA in host cells.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 26 citations in OpenAlex.
- Infection with Decapod iridescent virus 1: an emerging disease in shrimp culture.Archives of microbiology · 2022Review
- In situ loop-mediated isothermal amplification (LAMP) for identification of Plasmodium species in wide-range thin blood smears.Malaria journal · 2018Article
- A KDEL Retrieval System for ER-Golgi Transport of Japanese Encephalitis Viral Particles.Viruses · 2016Article
- Article
- [Seroprevalence of antibodies against infectious pathogens relevant to pregnancy among healthcare workers].Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz · 2012Article
- Development and evaluation of a reverse transcription-loop-mediated isothermal amplification assay for rapid detection of enterovirus 71.Journal of clinical microbiology · 2011Article
- Autoimmunity-related demyelination in infection by Japanese encephalitis virus.Journal of biomedical science · 2011Article
- A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification.Virology journal · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClinical diagnosis of Japanese encephalitis is usually difficult due to non-specific signs at the early and acute stages of the infection. Virus isolation from peripheral blood is also not possible because of the short period and low level of transient viremia even in the acute stage of the disease. It is thus urgent to develop a feasible and convenient method for laboratory diagnosis of the infection.
objectivesTo establish a newly designed molecular approach that can be used to detect intracellular Japanese encephalitis viral RNA in host cells. STUDY
designThe method was firstly established and then was carried out to test its efficacy in cultured BHK-21 cells, subsequently in peripheral blood mononuclear cells (PBMCs) isolated from mice that have been inoculated with JE virus suspension.
resultsIn this study, in situ reverse-transcription loop-mediated isothermal amplification (in situ RT-LAMP) was established; which combines merits of recently developed loop-mediated isothermal amplification (LAMP) and in situ reverse-transcriptase polymerase chain reaction (in situ RT-PCR).
conclusionsThe newly designed method can detect viral RNAs in peripheral blood mononuclear cells (PBMCs) in a short time with high sensitivity and efficiency.
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