ArticleThe open microbiology journal2009
A new method for isolating host-independent variants of Bdellovibrio bacteriovorus using E. coli auxotrophs.
Article in The open microbiology journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Article
- Characterization of the first highly predatoryIranian journal of basic medical sciences · 2020Article
- Effect of Predatory Bacteria on Human Cell Lines.PloS one · 2016Article
- Susceptibility of Select Agents to Predation by Predatory Bacteria.Microorganisms · 2015Article
- In and out: an analysis of epibiotic vs periplasmic bacterial predators.The ISME journal · 2014Article
- Genomic insights into an obligate epibiotic bacterial predator: Micavibrio aeruginosavorus ARL-13.BMC genomics · 2011Article
- Identification of genes essential for prey-independent growth of Bdellovibrio bacteriovorus HD100.Journal of bacteriology · 2011Article
- Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bdellovibrios are Gram-negative bacteria that are characterized by predatory behavior. Although Bdellovibrios exhibit an obligatory parasitic life cycle, it is possible to isolate Bdellovibrio variants that no longer require host cells for their growth. In this study, a new method for isolating Bdellovibrio bacteriovorus host-independent (HI) variants was developed. Filtered B. bacteriovorus prey cells were cultured with E. coli diaminopimelic acid (DAP) auxotrophs as host cells. Thereafter, the lysate was plated on DAP minus media, allowing only HI colonies to develop. Using this method, we have isolated numerous HI variants and demonstrated that the emergence of HI variants may be occurring at a higher frequency than was previously suggested.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.