ArticleThe open microbiology journal2009
Proteome analysis of Leptospira interrogans virulent strain.
Article in The open microbiology journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Surface proteomics and label-free quantification of Leptospira interrogans serovar Pomona.PLoS neglected tropical diseases · 2021Article
- Applied Proteomics in 'One Health'.Proteomes · 2021Review
- A Review on Host-Frontiers in cellular and infection microbiology · 2021Review
- Leptospira interrogans outer membrane protein LipL21 is a potent inhibitor of neutrophil myeloperoxidase.Virulence · 2018Article
- Reverse Vaccinology: An Approach for Identifying Leptospiral Vaccine Candidates.International journal of molecular sciences · 2017Review
- Characterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins.International journal of proteomics · 2014Article
- Extracellular proteome analysis of Leptospira interrogans serovar Lai.Omics : a journal of integrative biology · 2013Article
- Interaction of Leptospira interrogans with human proteolytic systems enhances dissemination through endothelial cells and protease levels.Infection and immunity · 2013Article
- Malaria vaccines: high-throughput tools for antigens discovery with potential for their development.Colombia medica (Cali, Colombia) · 2013Review
- Leptospira interrogans enolase is secreted extracellularly and interacts with plasminogen.PloS one · 2013Article
- OmpL1 is an extracellular matrix- and plasminogen-interacting protein of Leptospira spp.Infection and immunity · 2012Article
- Methylation and in vivo expression of the surface-exposed Leptospira interrogans outer-membrane protein OmpL32.Microbiology (Reading, England) · 2012Article
- Evaluation of immunoprotective activity of six leptospiral proteins in the hamster model of leptospirosis.The open microbiology journal · 2012Article
- Plasminogen binding proteins and plasmin generation on the surface of Leptospira spp.: the contribution to the bacteria-host interactions.Journal of biomedicine & biotechnology · 2012Article
- Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601.Cell research · 2011Article
- The OmpL37 surface-exposed protein is expressed by pathogenic Leptospira during infection and binds skin and vascular elastin.PLoS neglected tropical diseases · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptospirosis is a worldwide zoonotic infection of human and veterinary concern. Caused by pathogenic spirochetes of the genus Leptospira, the disease presents greater incidence in tropical and subtropical regions. The identification of proteins that could be involved in the bacteria host interactions may facilitate the search for immune protective antigens. We report the proteomic analysis of Leptospira interrogans serovar Pomona virulent strain LPF cultured from kidney and liver of infected hamsters. Total protein extracts were separated by two-dimensional gel electrophoresis (2-DE), 895 spots were analyzed by MALDI-TOF mass spectrometry (MS), and 286 were identified as leptospiral proteins, corresponding to 108 distinct proteins. These proteins are allocated in all the bacterial cell compartments and are distributed in every functional category. Furthermore, the previously described, known outer membrane proteins, OmpL1, LipL21, LipL31, LipL32/Hap-1, LipL41, LipL45, LipL46, LruA/LipL71, and OmpA-like protein Loa22 were all recognized. Most importantly, this research work identified 27 novel leptospiral proteins annotated as hypothetical open reading frames (ORFs). We report for the first time an array of proteins of the Leptospira expressed by virulent, low-passage strain. We believe that our studies, together with the genome data will enlighten our understanding of the disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.