ArticleEMBO reports2009
The SPX domain of the yeast low-affinity phosphate transporter Pho90 regulates transport activity.
Article in EMBO reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- The SPX protein family in plants: from phosphate sensors to multifunctional signaling hubs.Stress biology · 2026Review
- Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol.eLife · 2025Article
- Coming to Terms: The Mechanisms of Overplus and Luxury Phosphorus Uptake for Polyphosphate Storage in Microalgae and Yeast.Biotechnology and bioengineering · 2025Review
- A linear pathway for inositol pyrophosphate metabolism revealed by 18O labeling and model reduction.PLoS computational biology · 2025Article
- GhSPX1s Interact with GhPHR1A and GhPHL1A in Regulating Phosphate Starvation Response in Cotton.Biology · 2025Article
- MicroRNAs as potent regulators in nitrogen and phosphorus signaling transduction and their applications.Stress biology · 2024Review
- Mining key genes associated with phosphorus deficiency through genome-wide identification and characterization of cucumber SPX family genes.BMC plant biology · 2024Article
- Milestones in understanding transport, sensing, and signaling of the plant nutrient phosphorus.The Plant cell · 2024Review
- Genetics and metabolic responses of Artemisia annua L to the lake of phosphorus under the sparingly soluble phosphorus fertilizer: evidence from transcriptomics analysis.Functional & integrative genomics · 2024Article
- Differential Responses ofPlants (Basel, Switzerland) · 2023Article
- Inositol pyrophosphates activate the vacuolar transport chaperone complex in yeast by disrupting a homotypic SPX domain interaction.Nature communications · 2023Article
- Article
- Role of transporters in regulating mammalian intracellular inorganic phosphate.Frontiers in pharmacology · 2023Review
- Interaction of calcium responsive proteins and transcriptional factors with the PHO regulon in yeasts and fungi.Frontiers in cell and developmental biology · 2023Review
- The Genetic Basis of Phosphorus Utilization Efficiency in Plants Provide New Insight into Woody Perennial Plants Improvement.International journal of molecular sciences · 2022Review
- Intracellular phosphate sensing and regulation of phosphate transport systems in plants.Plant physiology · 2021Review
- Genome-wide identification and characterization of SPX-domain-containing protein gene family inPeerJ · 2021Article
- Phosphate availability and ectomycorrhizal symbiosis with Pinus sylvestris have independent effects on the Paxillus involutus transcriptome.Mycorrhiza · 2021Article
- Inositol Pyrophosphate Pathways and Mechanisms: What Can We Learn from Plants?Molecules (Basel, Switzerland) · 2020Review
- Mobilization and Cellular Distribution of Phosphate in the DiatomFrontiers in plant science · 2020Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yeast has two phosphate-uptake systems that complement each other: the high-affinity transporters (Pho84 and Pho89) are active under phosphate starvation, whereas Pho87 and Pho90 are low-affinity transporters that function when phosphate is abundant. Here, we report new regulatory functions of the amino-terminal SPX domain of Pho87 and Pho90. By studying truncated versions of Pho87 and Pho90, we show that the SPX domain limits the phosphate-uptake velocity, suppresses phosphate efflux and affects the regulation of the phosphate signal transduction pathway. Furthermore, split-ubiquitin assays and co-immunoprecipitation suggest that the SPX domain of both Pho90 and Pho87 interacts physically with the regulatory protein Spl2. This work suggests that the SPX domain inhibits low-affinity phosphate transport through a physical interaction with Spl2.
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