ArticleMicrobiology (Reading, England)2009
Anchoring and length regulation of Porphyromonas gingivalis Mfa1 fimbriae by the downstream gene product Mfa2.
Article in Microbiology (Reading, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Species-Dependent Aggregation of Oral Bacteria Under Simulated Microgravity.Life (Basel, Switzerland) · 2026Article
- Cryo-EM structure of the native assembled Mfa type V pilus from the periodontal pathogen Porphyromonas gingivalis.Communications biology · 2026Article
- Identification of a LolB-like protein in Porphyromonas gingivalis reveals selective LolA-LolB pairing.Scientific reports · 2026Article
- Local and Systemic Effects ofMicroorganisms · 2023Review
- Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae inJournal of oral microbiology · 2023Article
- Article
- In Silico Study of Cell Surface Structures ofInternational journal of molecular sciences · 2022Article
- Review
- Statistical approaches for differential expression analysis in metatranscriptomics.Bioinformatics (Oxford, England) · 2021Article
- Porphyromonas gingivalis fimbrial protein Mfa5 contains a von Willebrand factor domain and an intramolecular isopeptide.Communications biology · 2021Article
- Diversity analysis of genes encoding Mfa1 fimbrial components in Porphyromonas gingivalis strains.PloS one · 2021Article
- Phylogenetic diversity inJournal of oral microbiology · 2020Article
- Structure of polymerized type V pilin reveals assembly mechanism involving protease-mediated strand exchange.Nature microbiology · 2020Article
- Roles of Porphyromonas gingivalis and its virulence factors in periodontitis.Advances in protein chemistry and structural biology · 2020Review
- The Distinct Immune-Stimulatory Capacities of Porphyromonas gingivalis Strains 381 and ATCC 33277 Are Determined by theInfection and immunity · 2019Article
- A Putative Type V Pilus Contributes to Bacteroides thetaiotaomicron Biofilm Formation Capacity.Journal of bacteriology · 2019Article
- Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells.BMC microbiology · 2019Article
- Structural and functional characterization of shaft, anchor, and tip proteins of the Mfa1 fimbria from the periodontal pathogen Porphyromonas gingivalis.Scientific reports · 2018Article
- Maturation of the Mfa1 Fimbriae in the Oral PathogenFrontiers in cellular and infection microbiology · 2018Article
- Genetic exchange and reassignment inJournal of oral microbiology · 2018Review
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12 authors.
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Abstract
Porphyromonas gingivalis, a causative agent of periodontitis, has at least two types of thin, single-stranded fimbriae, termed FimA and Mfa1 (according to the names of major subunits), which can be discriminated by filament length and by the size of their major fimbrilin subunits. FimA fimbriae are long filaments that are easily detached from cells, whereas Mfa1 fimbriae are short filaments that are tightly bound to cells. However, a P. gingivalis ATCC 33277-derived mutant deficient in mfa2, a gene downstream of mfa1, produced long filaments (10 times longer than those of the parent), easily detached from the cell surface, similar to FimA fimbriae. Longer Mfa1 fimbriae contributed to stronger autoaggregation of bacterial cells. Complementation of the mutant with the wild-type mfa2 allele in trans restored the parental phenotype. Mfa2 is present in the outer membrane of P. gingivalis, but does not co-purify with the Mfa1 fimbriae. However, co-immunoprecipitation demonstrated that Mfa2 and Mfa1 are associated with each other in whole P. gingivalis cells. Furthermore, immunogold microscopy, including double labelling, confirmed that Mfa2 was located on the cell surface and likely associated with Mfa1 fimbriae. Mfa2 may therefore play a role as an anchor for the Mfa1 fimbriae and also as a regulator of Mfa1 filament length. Two additional downstream genes (pgn0289 and pgn0290) are co-transcribed with mfa1 (pgn0287) and mfa2 (pgn0288), and proteins derived from pgn0289, pgn0290 and pgn0291 appear to be accessory fimbrial components.
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