ArticleJournal of physiology and biochemistry2009
High fat diet-induced obesity modifies the methylation pattern of leptin promoter in rats.
Article in Journal of physiology and biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
91 citing papers in PubMed, 215 citations in OpenAlex.
- Trial
- Adherence to Mediterranean diet is associated with methylation changes in inflammation-related genes in peripheral blood cells.Journal of physiology and biochemistry · 2016Trial
- Effects of short-term high-fat overfeeding on genome-wide DNA methylation in the skeletal muscle of healthy young men.Diabetologia · 2012Trial
- Article
- Article
- Methylation status of leptin gene promoter in relatively lean Chinese adults with prediabetes and type 2 diabetes mellitus.World journal of diabetes · 2025Article
- Dysregulated Intestinal Nutrient Absorption in Obesity Is Associated with Altered Chromatin Accessibility.Organoids · 2025Article
- Innovative Therapeutic Approaches Targeting Obesity: Can Flavonoids Improve the Efficacy of Anti-Obesogenic Drugs?International journal of molecular sciences · 2025Review
- Alterations in DNA methylation machinery in a rat model of osteoarthritis of the hip.Journal of orthopaedic surgery and research · 2024Article
- The role of epigenetic mechanisms in the long-term effects of early-life adversity and mother-infant relationship on physiology and behavior of offspring in laboratory rats and mice.Developmental psychobiology · 2024Review
- Epigenetics in obesity: Mechanisms and advances in therapies based on natural products.Pharmacology research & perspectives · 2024Review
- Transcriptional and epigenetic changes after dietary and surgical weight loss interventions in an animal model of obesity.International journal of obesity (2005) · 2024Article
- Genetics of diabetes.World journal of diabetes · 2023Review
- Epigenetic Regulation of Obesity-Associated Type 2 Diabetes.Medicina (Kaunas, Lithuania) · 2022Review
- Fish oil supplementation of rats fed a high fat diet during pregnancy improves offspring insulin sensitivity.Frontiers in nutrition · 2022Article
- Epigenetic Regulation in Knee Osteoarthritis.Frontiers in genetics · 2022Review
- Brown Fat Dnmt3b Deficiency Ameliorates Obesity in Female Mice.Life (Basel, Switzerland) · 2021Article
- Histone H4 lysine 16 acetylation controls central carbon metabolism and diet-induced obesity in mice.Nature communications · 2021Article
- Article
- STAT3 phosphorylation in central leptin resistance.Nutrition & metabolism · 2021Review
31 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptin is an adipokine involved in body weight and food intake regulation whose promoter region presents CpG islands that could be subject to dynamic methylation. This methylation process could be affected by environmental (e.g. diet) or endogenous (e.g., adipocyte differentiation, inflammation, hypoxia) factors, and could influence adipocyte leptin gene expression. The aim of this article was to study whether a high-energy diet may affect leptin gene promoter methylation in rats. A group of eleven male Wistar rats were assigned into two dietary groups, one fed on a control diet for 11 weeks and the other on a high-fat cafeteria diet. Rats fed a high-energy diet become overweight and hyperleptinemic as compared to the controls. DNA isolated from retroperitoneal adipocytes was treated with bisulfite and a distal portion of leptin promoter (from -694 to -372 bp) including 13 CpG sites was amplified by PCR and sequenced. The studied promoter portion was slightly more methylated in the cafeteria-fed animals, which was statistically significant (p < 0.05) for one of the CpG sites (located at the position -443). In obese rats, such methylation was associated to lower circulating leptin levels, suggesting that this position could be important in the regulation of leptin gene expression, probably by being a target sequence of different transcription factors. Our findings reveal, for the first time, that leptin methylation pattern can be influenced by diet-induced obesity, and suggest that epigenetic mechanisms could be involved in obesity by regulating the expression of important epiobesigenic genes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.