Evidence map›Paper›PMID 19588255›Full record

ArticleGenetica2009

Microsatellite marker-mediated analysis of the EMBRAPA Rice Core Collection genetic diversity.

Tereza Cristina de Oliveira Borba, Rosana Pereira Vianello Brondani, Paulo Hideo Nakano Rangel, Claudio Brondani

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Article in Genetica, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

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  19. Development and application of a whole-genome simple sequence repeat panel for high-throughput genotyping in soybean.DNA research : an international journal for rapid publication of reports on genes and genomes · 2011
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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Tereza Cristina de Oliveira BorbaEscola de Agronomia e Engenharia de Alimentos, Universidade Federal de Goiás, Goiânia, GO, Brazil.
Rosana Pereira Vianello Brondani
Paulo Hideo Nakano Rangel
Claudio Brondani

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The objectives of this study were to determine the genetic structure of 242 accessions from the EMBRAPA Rice Core Collection (ERiCC), to create a mini-core collection and to develop a multiplex panel of fluorescent labeled simple sequence repeats (SSRs). Eighty-six SSRs were used to identify 1,066 alleles, with an average number of 12.4 alleles/locus and average polymorphism information content (PIC)/locus of 0.75. A model-based clustering method recognized the structure of the accessions on two levels, according to their cultivation system and origin. The most divergent subgroup identified was the worldwide lowland accessions, with the highest values for gene diversity (0.75), average Rogers distance modified by Wright (0.80), average number of alleles/locus (11.7) and private alleles (132). A mini-core was assembled with the most divergent 24 lowland and upland accessions. This mini-core displayed an average distance of 0.86, an average number of alleles/locus of 8.4 and an average PIC/locus of 0.8. From the 86 SSRs, 24 were selected to compose six multiplex panels in order to optimize the process of rice genotyping. This set of markers distinguished all 242 accessions, and showed an average PIC of 0.80 and an average number of alleles/locus of 15.4, higher than the entire set of 86 SSRs. Since the heterogeneity found in lines and cultivars of ERiCC was higher than expected, it is necessary to analyze pooled DNA samples to get a better estimate of genetic variability. The SSR characterization of ERiCC clearly indicates that there is high genetic variability in rice accessions stored in genebanks worldwide which can be promptly explored by rice pre-breeding programs.

Indexed as

Chromosomes, PlantCrosses, GeneticCytogenetic AnalysisDatabases, GeneticGenetic SpeciationGenetic VariationMicrosatellite RepeatsOryzaPhylogeny

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.