ArticlePlant physiology2009
Null mutation of the MdACS3 gene, coding for a ripening-specific 1-aminocyclopropane-1-carboxylate synthase, leads to long shelf life in apple fruit.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 111 citations in OpenAlex.
- Regulation of ethyl ester synthesis in two apple (Food chemistry. Molecular sciences · 2025Article
- The triggering mechanism for predominant hormonal signal production in fleshy fruit ripening.Molecular horticulture · 2025Review
- Genome-Wide Identification and Expression Analysis of 1-Aminocyclopropane-1-Carboxylate Synthase (ACS) Gene Family inInternational journal of molecular sciences · 2025Article
- An InDel variant in the promoter of the NAC transcription factor MdNAC18.1 plays a major role in apple fruit ripening.The Plant cell · 2024Article
- MdWRKY31-MdNAC7 regulatory network: orchestrating fruit softening by modulating cell wall-modifying enzyme MdXTH2 in response to ethylene signalling.Plant biotechnology journal · 2024Article
- Heterologous Overexpression of ApplePlants (Basel, Switzerland) · 2023Article
- A long noncoding RNA functions in high-light-induced anthocyanin accumulation in apple by activating ethylene synthesis.Plant physiology · 2022Article
- SnRK2 subfamily I protein kinases regulate ethylene biosynthesis by phosphorylating HB transcription factors to induce ACO1 expression in apple.The New phytologist · 2022Article
- Ethylene response factor MdERF4 and histone deacetylase MdHDA19 suppress apple fruit ripening through histone deacetylation of ripening-related genes.Plant physiology · 2022Article
- Duplication and Specialization of NUDX1 in Rosaceae Led to Geraniol Production in Rose Petals.Molecular biology and evolution · 2022Article
- Recent Advances in Phytohormone Regulation of Apple-Fruit Ripening.Plants (Basel, Switzerland) · 2021Review
- Role of MdERF3 and MdERF118 natural variations in apple flesh firmness/crispness retainability and development of QTL-based genomics-assisted prediction.Plant biotechnology journal · 2021Article
- Auxin-activated MdARF5 induces the expression of ethylene biosynthetic genes to initiate apple fruit ripening.The New phytologist · 2020Article
- Auxin Response Factor 2A Is Part of the Regulatory Network Mediating Fruit Ripening Through Auxin-Ethylene Crosstalk in Durian.Frontiers in plant science · 2020Article
- Red to Brown: An Elevated Anthocyanic Response in Apple Drives Ethylene to Advance Maturity and Fruit Flesh Browning.Frontiers in plant science · 2019Article
- GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene.Plant cell reports · 2018Article
- A Genome-Wide Association Study of Apple Quality and Scab Resistance.The plant genome · 2018Article
- Enhancement of apple coloration using jasmonate treatment without sacrificing storage potential.Plant signaling & behavior · 2018Article
- The Jasmonate-Activated Transcription Factor MdMYC2 RegulatesThe Plant cell · 2017Article
- Genome-wide association study unravels the genetic control of the apple volatilome and its interplay with fruit texture.Journal of experimental botany · 2017Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of MdACS1, coding for 1-aminocyclopropane-1-carboxylate synthase (ACS), parallels the level of ethylene production in ripening apple (Malus domestica) fruit. Here we show that expression of another ripening-specific ACS gene (MdACS3) precedes the initiation of MdACS1 expression by approximately 3 weeks; MdACS3 expression then gradually decreases as MdACS1 expression increases. Because MdACS3 expression continues in ripening fruit treated with 1-methylcyclopropene, its transcription appears to be regulated by a negative feedback mechanism. Three genes in the MdACS3 family (a, b, and c) were isolated from a genomic library, but two of them (MdACS3b and MdACS3c) possess a 333-bp transposon-like insertion in their 5' flanking region that may prevent transcription of these genes during ripening. A single nucleotide polymorphism in the coding region of MdACS3a results in an amino acid substitution (glycine-289 --> valine) in the active site that inactivates the enzyme. Furthermore, another null allele of MdACS3a, Mdacs3a, showing no ability to be transcribed, was found by DNA sequencing. Apple cultivars homozygous or heterozygous for both null allelotypes showed no or very low expression of ripening-related genes and maintained fruit firmness. These results suggest that MdACS3a plays a crucial role in regulation of fruit ripening in apple, and is a possible determinant of ethylene production and shelf life in apple fruit.
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