ArticleJournal of virology2009
A one-plasmid system to generate influenza virus in cultured chicken cells for potential use in influenza vaccine.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- New characteristics of cancer immunotherapy: trends in viral tumor immunotherapy with influenza virus-based approaches.Journal of Zhejiang University. Science. B · 2025Review
- An improved system to generate recombinant canine distemper virus.BMC veterinary research · 2024Article
- Synthetic Cell Lines for Inducible Packaging of Influenza A Virus.ACS synthetic biology · 2024Article
- Segmented, Negative-Sense RNA Viruses of Humans: Genetic Systems and Experimental Uses of Reporter Strains.Annual review of virology · 2023Review
- Antiviral options and therapeutics against influenza: history, latest developments and future prospects.Frontiers in cellular and infection microbiology · 2023Review
- Influenza Reverse Genetics-Historical Perspective.Cold Spring Harbor perspectives in medicine · 2021Review
- Development and application of reverse genetic technology for the influenza virus.Virus genes · 2021Review
- The Influenza B Virus Hemagglutinin Head Domain Is Less Tolerant to Transposon Mutagenesis than That of the Influenza A Virus.Journal of virology · 2018Article
- Article
- Alternative reverse genetics system for influenza viruses based on a synthesized swine 45S rRNA promoter.Virus genes · 2017Article
- Efficient size-independent chromosome delivery from yeast to cultured cell lines.Nucleic acids research · 2017Article
- Establishment of different plasmid only-based reverse genetics systems for the recovery of African horse sickness virus.Virology · 2016Article
- Efficient generation of influenza virus with a mouse RNA polymerase I-driven all-in-one plasmid.Virology journal · 2015Article
- Construction of Escherichia coli Mutant with Decreased Endotoxic Activity by Modifying Lipid A Structure.Marine drugs · 2015Article
- Generation of influenza virus from avian cells infected by Salmonella carrying the viral genome.PloS one · 2015Article
- All-in-one bacmids: an efficient reverse genetics strategy for influenza A virus vaccines.Journal of virology · 2014Article
- Traditional and new influenza vaccines.Clinical microbiology reviews · 2013Review
- Many ways to make an influenza virus--review of influenza virus reverse genetics methods.Influenza and other respiratory viruses · 2013Review
- Effect of deletion of genes involved in lipopolysaccharide core and O-antigen synthesis on virulence and immunogenicity of Salmonella enterica serovar typhimurium.Infection and immunity · 2011Article
- Improving Salmonella vector with rec mutation to stabilize the DNA cargoes.BMC microbiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Influenza virus has a set of ribonucleoproteins (RNPs) consisting of viral RNAs, influenza virus polymerase subunits, and nucleoprotein. Intracellular reconstitution of the whole set of RNPs via plasmid transfection results in the generation of influenza virus. By the use of reverse genetics and dual promoters, we constructed a 23.6-kb eight-unit plasmid that contains all the required constituents to generate influenza virus in chicken cells. Our "one-plasmid" system generated higher titers of influenza virus in chicken cells than the "eight-plasmid" system, enabling a simpler approach for generating vaccine seeds. Our study identified plasmid size as a potential limiting factor affecting transfection efficiency and hence the influenza viral yield from chicken cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.