ArticleThe Journal of biological chemistry2009
Two D-2-hydroxy-acid dehydrogenases in Arabidopsis thaliana with catalytic capacities to participate in the last reactions of the methylglyoxal and beta-oxidation pathways.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed, 130 citations in OpenAlex.
- Lysine Biosynthesis Defines a Metabolic Checkpoint for Gibberellin-Mediated Growth in Arabidopsis thaliana.Plant, cell & environment · 2026Article
- Seed priming to induce seed germination, seedling growth and enzymatic activity in watermelon.Die Naturwissenschaften · 2026Article
- Phenotypic and biochemical responses of high-yield tomato varieties to salinity and drought stress.PloS one · 2026Article
- Shotgun metagenomic mining reveals a new FAD-dependent D-lactate dehydrogenase in an isopod gut microbiome.Applied and environmental microbiology · 2025Article
- Implication ofInternational journal of molecular sciences · 2025Article
- Natural variation in Arabidopsis thaliana highlights a key role of glyoxalase I;2 in detoxifying glucose-derived reactive carbonyl species.BMC plant biology · 2025Article
- The branched-chain amino acid-related isoleucic acid: recent research advances.Plant biology (Stuttgart, Germany) · 2025Review
- Genomic identification, characterization, and stress-induced expression profiling of glyoxalase and D-lactate dehydrogenase gene families in Capsicum annuum.BMC plant biology · 2024Article
- Comparative proteomic and metabolomic analyses reveal stress responses of hemp to salinity.Plant cell reports · 2024Article
- PMF-seq: a highly scalable screening strategy for linking genetics to mitochondrial bioenergetics.Nature metabolism · 2024Article
- The D-lactate enigma: exploring the inflammatory influence of D-lactate in cattle.Frontiers in veterinary science · 2024Review
- Using UAV-Based Temporal Spectral Indices to Dissect Changes in the Stay-Green Trait in Wheat.Plant phenomics (Washington, D.C.) · 2024Article
- Article
- Lactate dehydrogenase D is a general dehydrogenase for D-2-hydroxyacids and is associated with D-lactic acidosis.Nature communications · 2023Article
- Proline, Cysteine and Branched-Chain Amino Acids in Abiotic Stress Response of Land Plants and Microalgae.Plants (Basel, Switzerland) · 2023Review
- The diversity of substrates for plant respiration and how to optimize their use.Plant physiology · 2023Review
- Viridiplantae-specific GLXI and GLXII isoforms co-evolved and detoxify glucosone in planta.Plant physiology · 2023Article
- Glyoxalase I activity affects Arabidopsis sensitivity to ammonium nutrition.Plant cell reports · 2022Article
- A Selective Fluorescent l-Lactate Biosensor Based on an l-Lactate-Specific Transcription Regulator and Förster Resonance Energy Transfer.Biosensors · 2022Article
- Revealing a New Family of D-2-Hydroxyglutarate Dehydrogenases inMicroorganisms · 2022Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis thaliana locus At5g06580 encodes an ortholog to Saccharomyces cerevisiae d-lactate dehydrogenase (AtD-LDH). The recombinant protein is a homodimer of 59-kDa subunits with one FAD per monomer. A substrate screen indicated that AtD-LDH catalyzes the oxidation of d- and l-lactate, d-2-hydroxybutyrate, glycerate, and glycolate using cytochrome c as an electron acceptor. AtD-LDH shows a clear preference for d-lactate, with a catalytic efficiency 200- and 2000-fold higher than that for l-lactate and glycolate, respectively, and a K(m) value for d-lactate of approximately 160 microm. Knock-out mutants showed impaired growth in the presence of d-lactate or methylglyoxal. Collectively, the data indicated that the protein is a d-LDH that participates in planta in the methylglyoxal pathway. Web-based bioinformatic tools revealed the existence of a paralogous protein encoded by locus At4g36400. The recombinant protein is a homodimer of 61-kDa subunits with one FAD per monomer. A substrate screening revealed highly specific d-2-hydroxyglutarate (d-2HG) conversion in the presence of an organic cofactor with a K(m) value of approximately 580 microm. Thus, the enzyme was characterized as a d-2HG dehydrogenase (AtD-2HGDH). Analysis of knock-out mutants demonstrated that AtD-2HGDH is responsible for the total d-2HGDH activity present in A. thaliana. Gene coexpression analysis indicated that AtD-2HGDH is in the same network as several genes involved in beta-oxidation and degradation of branched-chain amino acids and chlorophyll. It is proposed that AtD-2HGDH participates in the catabolism of d-2HG most probably during the mobilization of alternative substrates from proteolysis and/or lipid degradation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.