ArticleBMC research notes2009
Tousled kinase TLK1B counteracts the effect of Asf1 in inhibition of histone H3-H4 tetramer formation.
Article in BMC research notes, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- Chromatin looping-based CRISPR screen identifies TLK2 as chromatin loop formation regulator in cancer stemness plasticity.Nature communications · 2025Article
- Identification of the proteome complement of humanTLK1 reveals it binds and phosphorylates NEK1 regulating its activity.Cell cycle (Georgetown, Tex.) · 2017Article
- Tousled-like kinases phosphorylate Asf1 to promote histone supply during DNA replication.Nature communications · 2014Article
- Phenothiazine Inhibitors of TLKs Affect Double-Strand Break Repair and DNA Damage Response Recovery and Potentiate Tumor Killing with Radiomimetic Therapy.Genes & cancer · 2013Article
- The Tousled-Like Kinases as Guardians of Genome Integrity.ISRN molecular biology · 2012Article
- Adenoviral delivery of Tousled kinase for the protection of salivary glands against ionizing radiation damage.Gene therapy · 2011Article
- Exposure to acute hypoxia induces a transient DNA damage response which includes Chk1 and TLK1.Cell cycle (Georgetown, Tex.) · 2010Article
- Tousled kinase TLK1B mediates chromatin assembly in conjunction with Asf1 regardless of its kinase activity.BMC research notes · 2010Article
- TLK1B promotes repair of DSBs via its interaction with Rad9 and Asf1.BMC molecular biology · 2009Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Tousled-like kinases (TLKs) function in processes of chromatin assembly, including replication, transcription, repair, and chromosome segregation. TLK1 interacts specifically with the chromatin assembly factor Asf1, a histone H3-H4 chaperone, and with Rad9, a protein involved in DNA repair. Asf1 binds to the H3-H4 dimer at the same interface that is used for formation of the core tetramer, and hence Asf1 is implicated in disruption of the tetramer during transcription, although Asf1 also has a function in chromatin assembly during replication and repair.
findingsWe have used protein crosslinking with purified components to probe the interaction between H3, H4, Asf1, and TLK1B. We found that TLK1B, by virtue of its binding to Asf1, can restore formation of H3-H4 tetramers that is sterically prevented by adding Asf1.
conclusionWe suggest that TLK1B binds to Asf1 in a manner that interferes with its binding to the H3-H4 dimer, thereby allowing for H3-H4 tetramerization. A description of the function of TLK1 and Asf1 in chromatin remodeling is presented.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.