ArticlePloS one2009
Characterization of voltage-gated potassium channels in human neural progenitor cells.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- A conserved bioelectrical signature defines subventricular zone-derived human fetal neural stem cells and tracks their differentiation state.Frontiers in cell and developmental biology · 2026Article
- Evaluating chemical effects on human neural cells through calcium imaging and deep learning.iScience · 2024Article
- TLR4 activation inhibits the proliferation and osteogenic differentiation of skeletal muscle stem cells by downregulating LGI1.Journal of physiology and biochemistry · 2022Article
- Article
- Human cerebral spheroids undergo 4-aminopyridine-induced, activity associated changes in cellular composition and microrna expression.Scientific reports · 2022Article
- Engineering Tissues of the Central Nervous System: Interfacing Conductive Biomaterials with Neural Stem/Progenitor Cells.Advanced healthcare materials · 2022Review
- First Evidence of Kv3.1b Potassium Channel Subtype Expression during Neuronal Serotonergic 1C11 Cell Line Development.International journal of molecular sciences · 2020Article
- High-content imaging of 3D-cultured neural stem cells on a 384-pillar plate for the assessment of cytotoxicity.Toxicology in vitro : an international journal published in association with BIBRA · 2020Article
- Connexin Signaling Is Involved in the Reactivation of a Latent Stem Cell Niche after Spinal Cord Injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Neuropharmacological and Neurogenetic Correlates of Opioid Use Disorder (OUD) As a Function of Ethnicity: Relevance to Precision Addiction Medicine.Current neuropharmacology · 2020Review
- Reply to Meriney and Lacomis: Comment on direct aminopyridine effects on voltage-gated CaThe Journal of biological chemistry · 2018Article
- Loss of Function of KCNC1 is associated with intellectual disability without seizures.European journal of human genetics : EJHG · 2017Article
- Trace elements during primordial plexiform network formation in human cerebral organoids.PeerJ · 2017Article
- Electromagnetic Fields for the Regulation of Neural Stem Cells.Stem cells international · 2017Review
- Keppen-Lubinsky syndrome is caused by mutations in the inwardly rectifying K+ channel encoded by KCNJ6.American journal of human genetics · 2015Article
- Genetics studies indicate that neural induction and early neuronal maturation are disturbed in autism.Frontiers in cellular neuroscience · 2014Article
- Kv3.1 channels stimulate adult neural precursor cell proliferation and neuronal differentiation.The Journal of physiology · 2013Article
- Human glioma-initiating cells show a distinct immature phenotype resembling but not identical to NG2 glia.Journal of neuropathology and experimental neurology · 2013Article
- Neurogenesis and increase in differentiated neural cell survival via phosphorylation of Akt1 after fluoxetine treatment of stem cells.BioMed research international · 2013Article
- Voltage-gated K+ channels in adipogenic differentiation of bone marrow-derived human mesenchymal stem cells.Acta pharmacologica Sinica · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVoltage-gated potassium (K(v)) channels are among the earliest ion channels to appear during brain development, suggesting a functional requirement for progenitor cell proliferation and/or differentiation. We tested this hypothesis, using human neural progenitor cells (hNPCs) as a model system. METHODOLOGY/PRINCIPAL
findingsIn proliferating hNPCs a broad spectrum of K(v) channel subtypes was identified using quantitative real-time PCR with a predominant expression of the A-type channel K(v)4.2. In whole-cell patch-clamp recordings K(v) currents were separated into a large transient component characteristic for fast-inactivating A-type potassium channels (I(A)) and a small, sustained component produced by delayed-rectifying channels (I(K)). During differentiation the expression of I(A) as well as A-type channel transcripts dramatically decreased, while I(K) producing delayed-rectifiers were upregulated. Both K(v) currents were differentially inhibited by selective neurotoxins like phrixotoxin-1 and alpha-dendrotoxin as well as by antagonists like 4-aminopyridine, ammoniumchloride, tetraethylammonium chloride and quinidine. In viability and proliferation assays chronic inhibition of the A-type currents severely disturbed the cell cycle and precluded proper hNPC proliferation, while the blockade of delayed-rectifiers by alpha-dendrotoxin increased proliferation. CONCLUSIONS/SIGNIFICANCE: These findings suggest that A-type potassium currents are essential for proper proliferation of immature multipotent hNPCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.