ArticleInternational journal of plant genomics2009
Agrobacterium-mediated gene transfer to cereal crop plants: current protocols for barley, wheat, triticale, and maize.
Article in International journal of plant genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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Who cites it
68 citing papers in PubMed.
- Cloning, Transformation, and Reporter Gene Analysis of the SalT Promoter in Barley (Hordeum vulgare).Molecular biotechnology · 2026Article
- Engineering an Exo70 integrated domain of a barley NLR for improved blast resistance.The Plant cell · 2026Article
- Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping.Nature plants · 2025Article
- Arabinoxylan-Based Bioplastic from Wheat Bran: A Promising Replacement for Synthetic Plastics.Polymers · 2025Article
- VULGARE ROW-TYPE SIX 5 binds to the promoter of tillering and floral homeotic genes to regulate their expression.Plant physiology · 2025Article
- The Magnaporthe oryzae effector Pwl2 alters HIPP43 localization to suppress host immunity.The Plant cell · 2025Article
- CLAVATA signalling shapes barley inflorescence by controlling activity and determinacy of shoot meristem and rachilla.Nature communications · 2025Article
- PHOTOPERIOD 1 enhances stress resistance and energy metabolism to promote spike fertility in barley under high ambient temperatures.Plant physiology · 2025Article
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- Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley.Plant molecular biology · 2024Article
- HOMEOBOX2, the paralog of SIX-ROWED SPIKE1/HOMEOBOX1, is dispensable for barley spikelet development.Journal of experimental botany · 2024Article
- Anatomical insights into the vascular layout of the barley rachis: implications for transport and spikelet connection.Annals of botany · 2024Article
- The improvement of the in vitro plant regeneration in barley with the epigenetic modifier of histone acetylation, trichostatin A.Journal of applied genetics · 2024Article
- early maturity 7 promotes early flowering by controlling the light input into the circadian clock in barley.Plant physiology · 2024Article
- Barley MLA3 recognizes the host-specificity effector Pwl2 from Magnaporthe oryzae.The Plant cell · 2024Article
- Efficient transformation and regeneration of transgenic plants in commercial cultivars of Citrus aurantifolia and Citrus sinensis.Transgenic research · 2023Article
- Multilayered regulation of developmentally programmed pre-anthesis tip degeneration of the barley inflorescence.The Plant cell · 2023Article
- SWEET11b transports both sugar and cytokinin in developing barley grains.The Plant cell · 2023Article
- Novel resistance to the Bymovirus BaMMV established by targeted mutagenesis of the PDIL5-1 susceptibility gene in barley.Plant biotechnology journal · 2023Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of powerful "omics" technologies has enabled researchers to identify many genes of interest for which comprehensive functional analyses are highly desirable. However, the production of lines which ectopically express recombinant genes, or those in which endogenous genes are knocked down via stable transformation, remains a major bottleneck for the association between genetics and gene function in monocotyledonous crops. Methods of effective DNA transfer into regenerable cells of immature embryos from cereals by means of Agrobacterium tumefaciens have been modified in a stepwise manner. The effect of particular improvement measures has often not been significantly evident, whereas their combined implementation has resulted in meaningful advances. Here, we provide updated protocols for the Agrobacterium-mediated generation of stably transgenic barley, wheat, triticale and maize. Based upon these methods, several hundred independent transgenic lines have been delivered, with efficiencies of inoculated embryos leading to stably transgenic plants reaching 86% in barley, 10% in wheat, 4% in triticale, and 24% in maize.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.