ArticleEuropean journal of human genetics : EJHG2010
Specific epigenetic alterations of IGF2-H19 locus in spermatozoa from infertile men.
Article in European journal of human genetics : EJHG, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
86 citing papers in PubMed, 2 syntheses or guidelines pooled it, 235 citations in OpenAlex.
- Relationship between degree of methylation of sperm long interspersed nuclear element-1 (LINE-1) gene and alteration of sperm parameters and age: a meta-regression analysis.Journal of assisted reproduction and genetics · 2024Pooled it
- H19 Sperm Methylation in Male Infertility: A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2023Pooled it
- Sperm Imprinted Gene Methylation and DNA Fragmentation in ICSI Outcomes: A Pilot Study.Epigenomes · 2026Article
- Comprehensive DNA methylation profiling of sperm in male partners of couples with unexplained recurrent pregnancy loss.Clinical epigenetics · 2025Article
- Increased Mitochondrial Common Deletion in Oligo-Astheno-Teratozoospermia Samples Is Not Correlated with Increased Global DNA Methylation: Unexpected Results for ATP Levels.International journal of fertility & sterility · 2025Article
- Unmasking the Epigenome: Insights into Testicular Cell Dynamics and Reproductive Function.International journal of molecular sciences · 2025Review
- The Study ofInternational journal of fertility & sterility · 2025Article
- Decoding the Epigenetics of Infertility: Mechanisms, Environmental Influences, and Therapeutic Strategies.Epigenomes · 2024Review
- Sperm epigenetics and male infertility: unraveling the molecular puzzle.Human genomics · 2024Review
- Epigenetic modulation of long noncoding RNA H19 in oral squamous cell carcinoma-A narrative review.Non-coding RNA research · 2024Review
- Article
- Coordinated alternation of DNA methylation and alternative splicing of PBRM1 affect bovine sperm structure and motility.Epigenetics · 2023Article
- A DNA Methylation Perspective on Infertility.Genes · 2023Review
- The role of long non-coding RNA H19 in infertility.Cell death discovery · 2023Review
- Towards a Multi-Omics of Male Infertility.The world journal of men's health · 2023Review
- Age-associated epigenetic changes in mammalian sperm: implications for offspring health and development.Human reproduction update · 2023Review
- The role of spermatozoa-zona pellucida interaction in selecting fertilization-competent spermatozoa in humans.Frontiers in endocrinology · 2023Article
- Sperm DNA methylation patterns at discrete CpGs and genes involved in embryonic development are related to bull fertility.BMC genomics · 2022Article
- Predicting male fertility from the sperm methylome: application to 120 bulls with hundreds of artificial insemination records.Clinical epigenetics · 2022Article
- Contemporary Use of ICSI and Epigenetic Risks to Future Generations.Journal of clinical medicine · 2022Review
26 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation marks, a key modification of imprinting, are erased in primordial germ cells and sex specifically re-established during gametogenesis. Abnormal epigenetic programming has been proposed as a possible mechanism compromising male fertility. We analysed by pyrosequencing the DNA methylation status of 47 CpGs located in differentially methylated regions (DMRs), the DMR0 and DMR2 of the IGF2 gene and in the 3rd and 6th CTCF-binding sites of the H19 DMR in human sperm from men with normal semen and patients with teratozoospermia (T) and/or oligo-astheno-teratozoospermia (OAT). All normal semen samples presented the expected high global methylation level for all CpGs analysed. In the teratozoospermia group, 11 of 19 patients presented a loss of methylation at variable CpG positions either in the IGF2 DMR2 or in both the IGF2 DMR2 and the 6th CTCF of the H19 DMR. In the OAT group, 16 of 22 patients presented a severe loss of methylation of the 6th CTCF, closely correlated with sperm concentration. The methylation state of DMR0 and of the 3rd CTCF was never affected by the pathological status of sperm samples. This study demonstrates that epigenetic perturbations of the 6th CTCF site of the H19 DMR might be a relevant biomarker for quantitative defects of spermatogenesis in humans. Moreover, we defined a methylation threshold sustaining the classification of patients in two groups, unmethylated and methylated. Using this new classification of patients, the observed intrinsic imprinting defects of spermatozoa appear not to impair significantly the outcome of assisted reproductive technologies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.