ArticleJournal of cell science2009
Dynamic localization of G proteins in Dictyostelium discoideum.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- A dynamic partitioning mechanism polarizes membrane protein distribution.Nature communications · 2023Article
- MAPK docking motif in the Dictyostelium Gα2 subunit is required for aggregation and transcription factor translocation.Cellular signalling · 2021Article
- Different Heterotrimeric G Protein Dynamics for Wide-Range Chemotaxis in Eukaryotic Cells.Frontiers in cell and developmental biology · 2021Review
- Structural basis of Gip1 for cytosolic sequestration of G protein in wide-range chemotaxis.Nature communications · 2018Article
- The cAMP-induced G protein subunits dissociation monitored in live Dictyostelium cells by BRET reveals two activation rates, a positive effect of caffeine and potential role of microtubules.Cellular signalling · 2018Article
- Quantitative imaging of Rac1 activity in Dictyostelium cells with a fluorescently labelled GTPase-binding domain from DPAKa kinase.Histochemistry and cell biology · 2016Article
- A Model for Direction Sensing in Dictyostelium discoideum: Ras Activity and Symmetry Breaking Driven by a Gβγ-Mediated, Gα2-Ric8 -- Dependent Signal Transduction Network.PLoS computational biology · 2016Article
- Heterotrimeric G-protein shuttling via Gip1 extends the dynamic range of eukaryotic chemotaxis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Gβ Regulates Coupling between Actin Oscillators for Cell Polarity and Directional Migration.PLoS biology · 2016Article
- Function and Regulation of Heterotrimeric G Proteins during Chemotaxis.International journal of molecular sciences · 2016Review
- The G alpha subunit Gα8 inhibits proliferation, promotes adhesion and regulates cell differentiation.Developmental biology · 2013Article
- Femtosecond laser machined microfluidic devices for imaging of cells during chemotaxis.Journal of laser applications · 2011Article
- The relationship between pulsatile GnRH secretion and cAMP production in immortalized GnRH neurons.American journal of physiology. Endocrinology and metabolism · 2011Article
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Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Extracellular stimuli exert their effects on eukaryotic cells via serpentine G-protein-coupled receptors and mediate a vast number of physiological responses. Activated receptors stimulate heterotrimeric G-proteins, consisting of three subunits, alpha, beta and gamma. In Dictyostelium discoideum, cAMP binds to the cAMP receptor cAR1, which is coupled to the heterotrimer containing the Galpha2 subunit. These studies provide in vivo evidence as to how receptors influence the localization of the G-protein complex prior to and after ligand binding. Previous work has shown that the state of the heterotrimer could be monitored by changes in fluorescence (or Förster) resonance energy transfer (FRET) between the alpha2- and beta-subunits of D. discoideum. We now report the kinetics of G-protein activation as a loss of FRET prior to and after cAMP addition by using total internal reflection fluorescence microscopy (TIRFM). We also performed photobleaching experiments to measure G-protein recovery times. Our data show that inactive and active G-proteins cycle between the cytosol and plasma membrane. These data suggest that cAR1 activation slows the membrane dissociation ('off') rate of the alpha2 subunit, while simultaneously promoting betagamma-subunit dissociation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.