ArticleApplied and environmental microbiology2009
Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed.
- Discovery and characterization of an enantioselective family VIII esterase from effluent treatment plant sludge metagenome.Scientific reports · 2025Article
- Elucidating the kinetics and mechanisms of tetramethrin biodegradation by the fungal strain Neocosmospora sp. AF3.Microbial cell factories · 2025Article
- Computational and biochemical characterization of the immobilized esterase of Salinicoccus roseus for pesticide degradation.Scientific reports · 2024Article
- Optimizing microbial strain selection for pyrethroid biodegradation in contaminated environments through a TOPSIS-based decision-making system.Scientific reports · 2024Article
- Whole-genome sequencing ofFrontiers in microbiology · 2024Article
- Microbial elimination of pyrethroids: specific strains and involved enzymes.Applied microbiology and biotechnology · 2022Review
- Glutathione Is Involved in the Reduction of Methylarsenate to Generate Antibiotic Methylarsenite in Enterobacter sp. Strain CZ-1.Applied and environmental microbiology · 2022Article
- Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture withJournal of microbiology and biotechnology · 2022Article
- Highly efficient degradation of cypermethrin by a co-culture ofFrontiers in microbiology · 2022Article
- Expression, characterization, and immobilization of a novel SGNH esterase Est882 and its potential for pyrethroid degradation.Frontiers in microbiology · 2022Article
- Evaluate the Toxicity of Pyrethroid Insecticide Cypermethrin before and after Biodegradation byPlants (Basel, Switzerland) · 2021Article
- Novel Mechanism and Kinetics of Tetramethrin Degradation Using an IndigenousInternational journal of molecular sciences · 2021Article
- Pollutant Degrading Enzyme: Catalytic Mechanisms and Their Expanded Applications.Molecules (Basel, Switzerland) · 2021Review
- Detoxification Esterase StrH Initiates Strobilurin Fungicide Degradation inApplied and environmental microbiology · 2021Article
- Pyrethroid Carboxylesterase PytH fromApplied and environmental microbiology · 2020Article
- Article
- Biodegradation of Allethrin by a Novel FungusMicroorganisms · 2020Article
- New Insights into the Microbial Degradation ofMicroorganisms · 2020Article
- Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway inMicroorganisms · 2020Article
- Variability of Gut Microbiota Across the Life Cycle ofFrontiers in microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel esterase gene, pytH, encoding a pyrethroid-hydrolyzing carboxylesterase was cloned from Sphingobium sp. strain JZ-1. The gene contained an open reading frame of 840 bp. Sequence identity searches revealed that the deduced enzyme shared the highest similarity with many alpha/beta-hydrolase fold proteins (20 to 24% identities). PytH was expressed in Escherichia coli BL21 and purified using Ni-nitrilotriacetic acid affinity chromatography. It was a monomeric structure with a molecular mass of approximately 31 kDa and a pI of 4.85. PytH was able to transform p-nitrophenyl esters of short-chain fatty acids and a wide range of pyrethroid pesticides, and isomer selectivity was not observed. No cofactors were required for enzyme activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.