ArticleApplied and environmental microbiology2009
Establishment of Agrobacterium tumefaciens-mediated transformation of an oleaginous fungus, Mortierella alpina 1S-4, and its application for eicosapentaenoic acid producer breeding.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 94 citations in OpenAlex.
- Article
- Terpenes modulate bacterial and fungal growth and sorghum rhizobiome communities.Microbiology spectrum · 2023Article
- Potential of Mortierellaceae for polyunsaturated fatty acids production: mini review.Biotechnology letters · 2023Review
- Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production inJournal of fungi (Basel, Switzerland) · 2023Article
- Microbes: A Hidden Treasure of Polyunsaturated Fatty Acids.Frontiers in nutrition · 2022Review
- Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Advances in improving the biotechnological application of oleaginous fungus Mortierella alpina.Applied microbiology and biotechnology · 2021Review
- Role of the mitochondrial citrate-oxoglutarate carrier in lipid accumulation in the oleaginous fungus Mortierella alpina.Biotechnology letters · 2021Article
- Research advances on arachidonic acid production by fermentation and genetic modification of Mortierella alpina.World journal of microbiology & biotechnology · 2021Review
- The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production.Frontiers in microbiology · 2021Review
- The Role of Glyceraldehyde-3-Phosphate Dehydrogenases in NADPH Supply in the Oleaginous Filamentous FungusFrontiers in microbiology · 2020Article
- Application of high EPA-producingRSC advances · 2018Article
- Arachidonic acid production by the oleaginous fungusJournal of advanced research · 2018Review
- The role of acyl-CoA thioesterase ACOT8I in mediating intracellular lipid metabolism in oleaginous fungus Mortierella alpina.Journal of industrial microbiology & biotechnology · 2018Article
- Characterization of an Omega-3 Desaturase FromFrontiers in microbiology · 2018Article
- Establishment of an efficient transformation system for Pleurotus ostreatus.World journal of microbiology & biotechnology · 2017Article
- Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina.BMC biotechnology · 2017Article
- Application of a ω-3 Desaturase with an Arachidonic Acid Preference to Eicosapentaenoic Acid Production inFrontiers in bioengineering and biotechnology · 2017Article
- A silver bullet in a golden age of functional genomics: the impact ofFungal biology and biotechnology · 2017Review
- Application of a delta-6 desaturase with α-linolenic acid preference on eicosapentaenoic acid production in Mortierella alpina.Microbial cell factories · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene manipulation tools for an arachidonic-producing filamentous fungus, Mortierella alpina 1S-4, have not been sufficiently developed. In this study, Agrobacterium tumefaciens-mediated transformation (ATMT) was investigated for M. alpina 1S-4 transformation, using the uracil-auxotrophic mutant (ura5(-) strain) of M. alpina 1S-4 as a host strain and the homologous ura5 gene as a selectable marker gene. Furthermore, the gene for omega3-desaturase, catalyzing the conversion of n-6 fatty acid to n-3 fatty acid, was overexpressed in M. alpina 1S-4 by employing the ATMT system. As a result, we revealed that the frequency of transformation surpassed 400 transformants/10(8) spores, most of the integrated T-DNA appeared as a single copy at a random position in chromosomal DNA, and most of the transformants (60 to 80%) showed mitotic stability. Moreover, the accumulation of n-3 fatty acid in transformants was observed under the conditions of optimal omega3-desaturase gene expression. In particular, eicosapentaenoic acid (20:5n-3), an end product of n-3 fatty acids synthesized in M. alpina 1S-4, reached a maximum of 40% of total fatty acids. In conclusion, the ATMT system was found to be effective and suitable for the industrial strain Mortierella alpina 1S-4 and will be a useful tool for basic mutagenesis research and for industrial breeding of this strain.
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