ArticleApplied and environmental microbiology2009
Tolerance of the Ralstonia eutropha class I polyhydroxyalkanoate synthase for translational fusions to its C terminus reveals a new mode of functional display.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
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- Ambient Temperature Stable, Scalable COVID-19 Polymer Particle Vaccines Induce Protective Immunity.Advanced healthcare materials · 2022Article
- Tailored Nanoparticles With the Potential to Reduce Ruminant Methane Emissions.Frontiers in microbiology · 2022Article
- Covalent Functionalization of Bioengineered Polyhydroxyalkanoate Spheres Directed by Specific Protein-Protein Interactions.Frontiers in bioengineering and biotechnology · 2020Article
- Genome characteristics dictate poly-R-(3)-hydroxyalkanoate production in Cupriavidus necator H16.World journal of microbiology & biotechnology · 2018Review
- Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions.Microbial cell factories · 2017Article
- Immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beads.Microbial biotechnology · 2017Article
- Engineering Mycobacteria for the Production of Self-Assembling Biopolyesters Displaying Mycobacterial Antigens for Use as a Tuberculosis Vaccine.Applied and environmental microbiology · 2017Article
- Bioengineering a bacterial pathogen to assemble its own particulate vaccine capable of inducing cellular immunity.Scientific reports · 2017Article
- Forizymes - functionalised artificial forisomes as a platform for the production and immobilisation of single enzymes and multi-enzyme complexes.Scientific reports · 2016Article
- Display of Antigens on Polyester Inclusions Lowers the Antigen Concentration Required for a Bovine Tuberculosis Skin Test.Clinical and vaccine immunology : CVI · 2016Article
- In vivo polyester immobilized sortase for tagless protein purification.Microbial cell factories · 2015Article
- Smart polyhydroxyalkanoate nanobeads by protein based functionalization.Nanomedicine : nanotechnology, biology, and medicine · 2015Review
- Bioengineering of bacteria to assemble custom-made polyester affinity resins.Applied and environmental microbiology · 2015Article
- Polyhydroyxalkanoate synthase fusions as a strategy for oriented enzyme immobilisation.Molecules (Basel, Switzerland) · 2014Review
- In vivo self-assembly of stable green fluorescent protein fusion particles and their uses in enzyme immobilization.Applied and environmental microbiology · 2014Article
- New skin test for detection of bovine tuberculosis on the basis of antigen-displaying polyester inclusions produced by recombinant Escherichia coli.Applied and environmental microbiology · 2014Article
- Bioengineering of bacterial polymer inclusions catalyzing the synthesis of N-acetylneuraminic acid.Applied and environmental microbiology · 2013Article
- Efficient production of active polyhydroxyalkanoate synthase in Escherichia coli by coexpression of molecular chaperones.Applied and environmental microbiology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, the class I polyhydroxyalkanoate synthase (PhaC) from Ralstonia eutropha was investigated regarding the functionality of its conserved C-terminal region and its ability to tolerate translational fusions to its C terminus. MalE, the maltose binding protein, and green fluorescent protein (GFP) were considered reporter proteins to be translationally fused to the C terminus. Interestingly, PhaC remained active only when a linker was inserted between PhaC and MalE, whereas MalE was not functional. However, the extension of the PhaC N terminus by 458 amino acid residues was required to achieve a functionality of MalE. These data suggested a positive interaction of the extended N terminus with the C terminus. To assess whether a linker and/or N-terminal extension is generally required for a functional C-terminal fusion, GFP was fused to the C terminus of PhaC. Both fusion partners were active without the requirement of a linker and/or N-terminal extension. A further reporter protein, the immunoglobulin G binding ZZ domain of protein A, was translationally fused to the N terminus of the fusion protein PhaC-GFP and resulted in a tripartite fusion protein mediating the production of polyester granules displaying two functional protein domains.
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