ArticleGenetics2009
Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- GBSC: graph-based sequence clustering method for similar short tandem repeats in protein sequences.Bioinformatics (Oxford, England) · 2026Article
- Intron-lariat spliceosomes convert lariats to true circles: implications for intron transposition.bioRxiv : the preprint server for biology · 2024Article
- A Non-Dicer RNase III and Four Other Novel Factors Required for RNAi-Mediated Transposon Suppression in the Human Pathogenic YeastG3 (Bethesda, Md.) · 2019Article
- Co-evolution of spliceosomal disassembly interologs: crowning J-protein component with moonlighting RNA-binding activity.Current genetics · 2019Article
- The roles of a flagellar HSP40 ensuring rhythmic beating.Molecular biology of the cell · 2019Article
- DNAJC17 is localized in nuclear speckles and interacts with splicing machinery components.Scientific reports · 2018Article
- Cwc23 is a component of the NTR complex and functions to stabilize Ntr1 and facilitate disassembly of spliceosome intermediates.Nucleic acids research · 2018Article
- Evolutionary Conservation and Emerging Functional Diversity of the Cytosolic Hsp70:J Protein Chaperone Network ofG3 (Bethesda, Md.) · 2017Article
- Multiple protein-protein interactions converging on the Prp38 protein during activation of the human spliceosome.RNA (New York, N.Y.) · 2016Article
- Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways.RNA biology · 2016Article
- Article
- Post-zygotic sterility and cytonuclear compatibility limits in S. cerevisiae xenomitochondrial cybrids.Frontiers in genetics · 2014Article
- Dissection of the factor requirements for spliceosome disassembly and the elucidation of its dissociation products using a purified splicing system.Genes & development · 2013Article
- DExD/H-box RNA helicases in ribosome biogenesis.RNA biology · 2013Review
- RNA helicases in splicing.RNA biology · 2013Review
- Assembling a protein-protein interaction map of the SSU processome from existing datasets.PloS one · 2011Article
- The function of the NineTeen Complex (NTC) in regulating spliceosome conformations and fidelity during pre-mRNA splicing.Biochemical Society transactions · 2010Review
- The HSP70 chaperone machinery: J proteins as drivers of functional specificity.Nature reviews. Molecular cell biology · 2010Review
- The ATPase and helicase activities of Prp43p are stimulated by the G-patch protein Pfa1p during yeast ribosome biogenesis.The EMBO journal · 2009Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Prp43p catalyzes essential steps in pre-mRNA splicing and rRNA biogenesis. In splicing, Spp382p stimulates the Prp43p helicase to dissociate the postcatalytic spliceosome and, in some way, to maintain the integrity of the spliceosome assembly. Here we present a dosage interference assay to identify Spp382p-interacting factors by screening for genes that when overexpressed specifically inhibit the growth of a conditional lethal prp38-1 spliceosome assembly mutant in the spp382-1 suppressor background. Identified, among others, are genes encoding the established splicing factors Prp8p, Prp9p, Prp11p, Prp39p, and Yhc1p and two poorly characterized proteins with possible links to splicing, Sqs1p and Cwc23p. Sqs1p copurifies with Prp43p and is shown to bind Prp43p and Spp382p in the two-hybrid assay. Overexpression of Sqs1p blocks pre-mRNA splicing and inhibits Prp43p-dependent steps in rRNA processing. Increased Prp43p levels buffer Sqs1p cytotoxicity, providing strong evidence that the Prp43p DExD/H-box protein is a target of Sqs1p. Cwc23p is the only known yeast splicing factor with a DnaJ motif characteristic of Hsp40-like chaperones. We show that similar to SPP382, CWC23 activity is critical for efficient pre-mRNA splicing and intron metabolism yet, surprisingly, this activity does not require the canonical DnaJ/Hsp40 motif. These and related data establish the value of this dosage interference assay for finding genes that alter cellular splicing and define Sqs1p and Cwc23p as prospective modulators of Spp382p-stimuated Prp43p function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.