ArticleThe Journal of cell biology2009
Organelle tethering by a homotypic PDZ interaction underlies formation of the Golgi membrane network.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 71 citations in OpenAlex.
- A distinct Golgi-targeting mechanism of dGM130 inFrontiers in molecular neuroscience · 2023Article
- Retro-2 alters Golgi structure.Scientific reports · 2022Article
- In-resin CLEM of Epon-embedded cells using proximity labeling.Scientific reports · 2022Article
- Measurement of Lysosome Positioning by Shell Analysis and Line Scan.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mechanisms and roles of mitochondrial localisation and dynamics in neuronal function.Neuronal signaling · 2020Review
- Golgin45-Syntaxin5 Interaction Contributes to Structural Integrity of the Golgi Stack.Scientific reports · 2019Article
- Detailed Analysis of the Interaction of Yeast COG Complex.Cell structure and function · 2018Article
- Structure function relations in PDZ-domain-containing proteins: Implications for protein networks in cellular signalling.Journal of biosciences · 2018Review
- CD36 mediates H2O2-induced calcium influx in lung microvascular endothelial cells.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Article
- Chaperoning SNARE assembly and disassembly.Nature reviews. Molecular cell biology · 2016Review
- GRASP: A Multitasking Tether.Frontiers in cell and developmental biology · 2016Review
- Induced oligomerization targets Golgi proteins for degradation in lysosomes.Molecular biology of the cell · 2015Article
- Structural basis for the interaction between the Golgi reassembly-stacking protein GRASP65 and the Golgi matrix protein GM130.The Journal of biological chemistry · 2015Article
- Synergistic action of dendritic mitochondria and creatine kinase maintains ATP homeostasis and actin dynamics in growing neuronal dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- A novel assay reveals preferential binding between Rabs, kinesins, and specific endosomal subpopulations.The Journal of cell biology · 2015Article
- GRASPs in Golgi Structure and Function.Frontiers in cell and developmental biology · 2015Review
- The role of Sec3p in secretory vesicle targeting and exocyst complex assembly.Molecular biology of the cell · 2014Article
- The centrosome-Golgi apparatus nexus.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Myristoylation restricts orientation of the GRASP domain on membranes and promotes membrane tethering.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Formation of the ribbon-like membrane network of the Golgi apparatus depends on GM130 and GRASP65, but the mechanism is unknown. We developed an in vivo organelle tethering assaying in which GRASP65 was targeted to the mitochondrial outer membrane either directly or via binding to GM130. Mitochondria bearing GRASP65 became tethered to one another, and this depended on a GRASP65 PDZ domain that was also required for GRASP65 self-interaction. Point mutation within the predicted binding groove of the GRASP65 PDZ domain blocked both tethering and, in a gene replacement assay, Golgi ribbon formation. Tethering also required proximate membrane anchoring of the PDZ domain, suggesting a mechanism that orientates the PDZ binding groove to favor interactions in trans. Thus, a homotypic PDZ interaction mediates organelle tethering in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.